Mycobacterium tuberculosis septum site determining protein, Ssd encoded by rv3660c, promotes filamentation and elicits an alternative metabolic and dormancy stress response
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Rv3660c encodes a septum site determining protein (Ssd) that inhibits septum formation when overexpressed
"Increased expression of ssd in M. smegmatis and M. tuberculosis inhibited septum formation resulting in elongated cells devoid of septa"
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Transposon disruption of rv3660c leads to minicell morphology
"Disruption of rv3660c by transposon insertion negated the unique transcriptional response and led to a reduced bacterial length"
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Ssd expression induces the dormancy regulon and alternative sigma factors
"Transcriptional mapping in M. tuberculosis showed that increased ssd expression elicited a unique response including the dormancy regulon and alternative sigma factors that are thought to play a role in adaptive metabolism"
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First link between septum regulation and dormancy/persistence response
"This study establishes the first connection between a septum regulatory protein and induction of alternative metabolism consisting of alternative sigma factors and the dormancy regulon that is associated with establishing a non-replicating persistent intracellular lifestyle"
Septum site placement in Mycobacteria - identification and characterisation of mycobacterial homologues of Escherichia coli MinD.
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Rv1708 (not Rv3660c) is the true E. coli MinD homologue in M. tuberculosis
"Comparative structural analyses showed Rv1708 to be closer in similarity to Ec MinD than Rv3660c"
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Rv3660c has an N-terminal region absent in E. coli MinD
"The N-terminal region encircled in Rv3660c is absent in E"
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The M. smegmatis Rv1708 orthologue MSMEG_3743 has confirmed ATPase activity
"MSMEG_3743 displayed ATPase activity, consistent with its containing a conserved Walker A motif"
Identification of cell cycle regulators in Mycobacterium tuberculosis by inhibition of septum formation and global transcriptional analysis
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Rv3660c is induced by FtsZ inhibitors albendazole and thiabendazole
"Inhibition of septum formation prevented cell division and led to bacterial filamentation...Microarray-based transcriptional profiling allowed the evaluation of multiple metabolic processes in response to inhibition of septum formation"
Genes required for mycobacterial growth defined by high density mutagenesis
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Rv3660c is essential for optimal growth of M. tuberculosis
"comprehensively identify the genes required by the causative agent, Mycobacterium tuberculosis, for optimal growth"
targetTB - a target identification pipeline for Mycobacterium tuberculosis through an interactome, reactome and genome-scale structural analysis
BioReason SFT reasoning trace for Rv3660c
Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence
Annotation inferences using phylogenetic trees