treSB curation notes

• Q88FN0 is a 1106-aa fusion with TreS/GH13 and maltokinase domain signatures. [file:PSEPK/treSB/treSB-uniprot.txt, "DR InterPro; IPR012810; TreS/a-amylase_N."] [file:PSEPK/treSB/treSB-uniprot.txt, "DR InterPro; IPR012811; TreS_maltokin_C_dom."]
• The TreS reaction is D-maltose to alpha,alpha-trehalose (EC 5.4.99.16; RHEA:15145). [file:PSEPK/treSB/treSB-uniprot.txt, "Reaction=D-maltose = alpha,alpha-trehalose"]
• The distinct maltokinase reaction is D-maltose + ATP to alpha-maltose 1-phosphate + ADP + H(+) (EC 2.7.1.175; RHEA:31915). [file:PSEPK/treSB/treSB-uniprot.txt, "Reaction=D-maltose + ATP = alpha-maltose 1-phosphate + ADP + H(+)"]
• The seeded GOA represents only the TreS activity. A NEW carbohydrate kinase annotation and a proposed substrate-specific maltokinase term were added so the fusion is not curated as monofunctional.
• PTHR10357 PAINT node PTN000039847 supports GO:0047471 in bacteria from the characterized Mycobacterium TreS seed P9WQ19. Q88FN0 and P9WQ19 are both verified members of PTHR10357:SF219. [file:interpro/panther/PTHR10357/PTHR10357-paint.tsv, "PTN000039847"] [file:interpro/panther/panther-members.tsv, "Q88FN0\tPTHR10357:SF219"]
• Mycobacterium experiments establish the conserved four-step TreS-Mak-GlgE-GlgB pathway, but they do not directly assay Q88FN0. PMID:20305657
• No target-specific biochemical publication was present in the local publication cache; this uncertainty is recorded on both core functions.
• The fused Mak reaction produces the alpha-maltose 1-phosphate consumed by adjacent GlgE, so the protein is annotated to alpha-glucan biosynthesis. The reversible TreS leaf is not independently assigned that process. [file:PSEPK/treSB/treSB-uniprot.txt, "Reaction=D-maltose + ATP = alpha-maltose 1-phosphate + ADP + H(+)"] [file:PSEPK/glgE/glgE-uniprot.txt, "Maltosyltransferase that uses maltose 1-phosphate (M1P)"]

OpenScientist reconciliation

Source: file:PSEPK/treSB/treSB-deep-research-openscientist.md (1,025.98 seconds).

• The report corroborates the bifunctional TreS-Mak fusion, both exact
  reactions, and placement immediately upstream of GlgE in the pathway.
• Its proposed catalytic-residue positions, sole-source and gatekeeper claims,
  operon interpretation, and transferred stress phenotypes are computational
  or ortholog-based inferences and were not promoted into the curated core
  functions.
• The report found no direct biochemical characterization of Q88FN0, so both
  catalytic assignments retain explicit target-specific knowledge gaps.