FCY1 (Q12178, YPR062W) notes

Module: pyrimidine_salvage (optional cytosine deamination step), role = cytosine deaminase (EC 3.5.4.1). YeastCyc: CYTDEAM-RXN in YEAST-RNT-SALV.

Evidence journal

• Activity and phenotype PMID:9000374
• Growth on cytosine PMID:9000374
• Limiting for cytidine use PMID:10501935
• Mechanism (computational) PMID:15535715; Zn, homodimer, many PDB structures [UniProt:Q12178]
• Yeast DRAP deaminase is Rib2 [UniProt RIB2: "Diaminohydroxyphosphoribosylaminopyrimidine deaminase"].

Decisions

• IBA GO:0008835 DRAP deaminase (RibD-only donor, deep CDA-superfamily node) REMOVE.
• IDA from PMID:15535715 (ONIOM computational study) ACCEPT on content but evidence code questionable.
• Cytidine metabolic process rows KEEP_AS_NON_CORE (acts on cytosine released from cytidine).
• GO-CAM YEAST-RNT-SALV types FCY1 with obsolete GO:0102480 (5-fluorocytosine deaminase), so no RCA cytosine deaminase row reaches GOA.