Gene Ontology annotation through association of InterPro records with GO terms
Electronic Gene Ontology annotations created by transferring manual GO annotations between related proteins based on shared sequence features
TreeGrafter-generated GO annotations
Combined Automated Annotation using Multiple IEA Methods
UniProtKB reviewed entry for thiI
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"Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs"
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"Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS."
QuickGO GOA annotations for thiI
PANTHER family metadata for thiI
Falcon deep research report: thiI (Q88CY4, PP_5045) in Pseudomonas putida KT2440
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"deletion of **thiI** causes a **drastic reduction of s4U in bulk tRNA**, directly supporting this assignment"
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"encodes a **tRNA sulfurtransferase (tRNA 4-thiouridine synthase; EC 2.8.1.4)** responsible for installing **4-thiouridine (s4U)** in cellular tRNA"
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"ATP-dependent thiolation of uridine in tRNA to form 4-thiouridine (s4U), typically at position 8"
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"the **THUMP domain captures the tRNA 3′-CCA terminus**, and proper tRNA architecture is essential for catalysis"
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"sulfur is mobilized from **L-cysteine by the cysteine desulfurase IscS**, transferred as a **persulfide** to ThiI, then inserted into tRNA"
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"a **ΔthiI** strain shows **drastically reduced** s4U relative to wild type"
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"the strongest direct evidence supports the **tRNA s4U pathway**, while any thiamine-biosynthesis role remains an inference from family knowledge rather than KT2440-specific proof"
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"The functional context is **intracellular/cytosolic**, acting on mature cellular tRNA"