Gene Ontology annotation through association of InterPro records with GO terms.

Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt.

GO_REF:0000104

Electronic Gene Ontology annotations created by transferring manual GO annotations between related proteins based on shared sequence features

GO_REF:0000117

Electronic Gene Ontology annotations created by ARBA machine learning models

GO_REF:0000118

Gene Ontology annotation by TreeGrafter/PANTHER phylogenetic inference.

GO_REF:0000120

Combined Automated Annotation using Multiple IEA Methods

PMID:16725371

Arabidopsis thaliana expresses two functional isoforms of Arvp, a protein involved in the regulation of cellular lipid homeostasis.

Arabidopsis ARV homologs complement yeast arv1 deficiency and localize to ER in onion cells; this does not resolve the Miscanthus fusion.

"both proteins are exclusively targeted to the endoplasmic reticulum."
Loss of the cysteine-rich ARV subdomain alone did not abolish homolog activity.

"Removal of the cysteine-rich subdomain has no effect on Arvp activity,"

PMID:33911077

Chromosome-scale assembly and analysis of biomass crop Miscanthus lutarioriparius genome.

M. lutarioriparius genome was assembled to chromosome scale from WGS data with contig N50 of 1.71 Mb covering 96.64% of the genome.

PMID:23668914

Arv1 regulates PM and ER membrane structure and homeostasis but is dispensable for intracellular sterol transport.

Yeast Arv1 affects ER and plasma-membrane organization rather than direct sterol transport between those membranes.

"We report that sterol transport between the ER and PM is unaffected by Arv1 deficiency."

file:9POAL/NCGR_LOCUS10166/NCGR_LOCUS10166-deep-research-manual.md

Manual deep research on NCGR_LOCUS10166 identifying gene prediction artifact.

file:9POAL/NCGR_LOCUS10166/NCGR_LOCUS10166-bioinformatics/RESULTS.md

Reproducible deposited sequence and gene-coordinate comparison for NCGR_LOCUS10166

The candidate retains HDH catalytic/zinc sites; separate HDH/ARV genes are adjacent on chromosome 1, while the candidate lies on chromosome 2. Actual fusion expression and targeting remain unresolved.

file:9POAL/NCGR_LOCUS10166/NCGR_LOCUS10166-hypotheses/fusion-model-arv-function-and-targeting/openscientist.md

OpenScientist focused report on the NCGR_LOCUS10166 HDH-ARV fusion and ARV sterol-transport scope

The HDH domain remains the defensible core activity, while ARV-derived direct sterol transport terms should be downgraded because the candidate fusion is probably a gene-model artifact and yeast Arv1 is dispensable for ER-PM sterol traffic.

"Recommend **removing or generalizing GO:0032366 (intracellular sterol transport)** and **GO:0097036 (regulation of PM sterol distribution)**"

file:9POAL/NCGR_LOCUS10166/NCGR_LOCUS10166-uniprot.txt

UniProt record for NCGR_LOCUS10166