Reconstitution and characterization of a new desosaminyl transferase, EryCIII, from the erythromycin biosynthetic pathway.
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EryCIII transfers TDP-D-desosamine onto alpha-mycarosyl erythronolide B to form erythromycin D, with high activity and donor/acceptor selectivity.
"EryCIII converts alpha-mycarosyl erythronolide B into erythromycin D using TDP-d-desosamine as the glycosyl donor."
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EryCIII is highly active on its own (kcat > 100 min-1), implying it does not require an auxiliary partner for catalysis.
"The enzyme was found to be highly active with a kcat greater than 100 min-1"
Structure of the glycosyltransferase EryCIII in complex with its activating P450 homologue EryCII.
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EryCIII, with its partner EryCII, attaches a nucleotide-activated sugar to the macrolide scaffold; the two form a heterotetramer.
"the glycosyltransferase (GT) EryCIII, in concert with its partner EryCII, attaches a nucleotide-activated sugar to the macrolide scaffold with high specificity"
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In this study purified EryCIII was inactive on its own and its GT activity was restored by EryCII, indicating the catalytic activity is realized in the EryCIII-EryCII complex.
"Purified EryCIII was used for in vitro glycosylation assays as described previously, but the enzyme was inactive under all conditions tried."
eryCIII review notes (BGC project) with predicted-complex evidence
Gene Ontology annotation through association of InterPro records with GO terms
Gene Ontology annotation based on Ensembl/EnsemblGenomes orthology