Gene Ontology annotation through association of InterPro records with GO terms

Annotation inferences using phylogenetic trees

Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt

GO_REF:0000117

Electronic Gene Ontology annotations created by ARBA machine learning models

GO_REF:0000120

Combined Automated Annotation using Multiple IEA Methods

PMID:11470797

Molecular cloning and characterization of a dermatan-specific N-acetylgalactosamine 4-O-sulfotransferase.

Human CHST14 catalyzes GalNAc C4 sulfation of dermatan; substrate specificity and type-II topology were distinguished experimentally versus computationally.

PMID:19199708

Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).

CHST14 was identified by two matching peptides in a human parotid exosome-rich fraction.

PMID:35156780

CFTR interactome mapping using the mammalian membrane two-hybrid high-throughput screening system.

Human CHST14 and CFTR have positive mammalian ubiquitin-reconstruction interaction records.

Reactome:R-HSA-2022063

CHST14 transfers SO4(2-) to GalNAc in dermatan or DS

CHST14 is explicitly modeled in Golgi dermatan sulfation, with normal and defective-mutant events distinguished.

Reactome:R-HSA-2022923

DS-GAG biosynthesis

CHST14 is explicitly modeled in Golgi dermatan sulfation, with normal and defective-mutant events distinguished.

Reactome:R-HSA-3636919

Defective CHST14 does not transfer SO4(2-) to GalNAc in dermatan or DS

CHST14 is explicitly modeled in Golgi dermatan sulfation, with normal and defective-mutant events distinguished.

PMID:12847091

Specificities of three distinct human chondroitin/dermatan N-acetylgalactosamine 4-O-sulfotransferases demonstrated using partially desulfated dermatan sulfate as an acceptor: implication of differential roles in dermatan sulfate biosynthesis.

Human D4ST1 preferentially sulfates GalNAc in iduronic-acid-containing sequences of partially desulfated dermatan.

PMID:19661164

Dermatan 4-O-sulfotransferase 1 is pivotal in the formation of iduronic acid blocks in dermatan sulfate.

CHST14 depletion reduces sulfated iduronic-acid blocks in human fibroblast dermatan sulfate.

PMID:20004762

Loss of dermatan-4-sulfotransferase 1 function results in adducted thumb-clubfoot syndrome.

Biallelic CHST14 variants cause a connective-tissue disorder; studied patient fibroblasts lack detectable newly synthesized dermatan sulfate and show increased chondroitin sulfate.

PMID:20533528

Loss-of-function mutations of CHST14 in a new type of Ehlers-Danlos syndrome.

CHST14 loss of function causes a form of Ehlers-Danlos syndrome associated with loss of decorin dermatan chains.

PMID:29976758

Dermatan sulfate epimerase 1 and dermatan 4-O-sulfotransferase 1 form complexes that generate long epimerized 4-O-sulfated blocks.

CHST14 and DS-epi1 cooperate to form long sulfated iduronic-acid blocks and associate in Golgi-localized cellular assays.

file:human/CHST14/CHST14-source-evidence.json

CHST14 source, assay and annotation provenance