Characterization of Candida albicans orthologue of the Saccharomyces cerevisiae signal-peptidase-subunit encoding gene SPC3.
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C. albicans SPC3 is the true fungal SPC3 ortholog, is essential for viability, and functionally connects the Candida signal peptidase complex to the ER Sec61 translocon.
"The cloned gene could rescue the phenotype associated with a spc3 mutation in S. cerevisiae"
The Role of Secretory Pathways in Candida albicans Pathogenesis.
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In C. albicans, signal peptidase cleavage follows ER translocation; Spc3p and Sec11p had been assigned as signal peptidase complex subunits, but SEC11 itself had not been directly characterized in C. albicans as of 2020.
"Following translocation into the ER, the N-terminal signal peptide is cleaved by signal peptidase"
Structure of the human signal peptidase complex reveals the determinants for signal peptide cleavage.
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Human SEC11A and SEC11C form the proteolytic subunits of two ER-localized signal peptidase complexes, with an active site that abuts the ER membrane.
"human SPC exists in two functional paralogs with distinct proteolytic subunits"
The Sec11 C-terminal short helix promotes productive engagement of internal signal sequences with the signal peptidase complex.
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S. cerevisiae Sec11 is the SPC catalytic subunit and its C-terminal short helix helps stabilize internal signal sequences during cleavage.
"Sec11, the catalytic subunit of the SPC, is evolutionarily conserved from bacteria to humans"
UniProtKB Q5A869 Candida albicans SEC11 flat file
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The Q5A869 record places Candida SEC11 in the peptidase S26B family and in PANTHER:PTHR10806:SF6.
"Belongs to the peptidase S26B family."
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UniProt annotates Candida SEC11 as a type-II ER membrane protein by similarity to S. cerevisiae SEC11.
"SUBCELLULAR LOCATION: Endoplasmic reticulum membrane"
PANTHER PTHR10806 PAINT IBD annotations
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PAINT places generic peptidase activity on PTN000086749 at the PTHR10806 root and places signal peptidase complex membership plus protein maturation on the eukaryotic PTN000086750 node.
"PTHR10806 PTN000086750 GO:0005787 C IBD false"
Gene Ontology annotation through association of InterPro records with GO terms
Use of the ND evidence code for Gene Ontology (GO) terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Combined Automated Annotation using Multiple IEA Methods