"Actin-RPV is a major component of the dynactin complex, an activator of dynein-driven vesicle movement, indicating that unlike conventional actins which work in conjunction with myosin motors, actin-RPV may be involved in cytoplasmic movements via a microtubule-based system."

"In contrast, Arp1A accumulated asymmetrically at the cell cortex during metaphase such that it is preferentially localized to the cortex that is distal to the mitotic spindle"

"we found that LGN was required for the cortical localization of dynein-dynactin"

"BCCIPα, but not BCCIPβ or GST itself, was sufficient to pull down the dynactin components, p150 glued and Arp-1"

"The isoforms were found in a constant ratio of approximately 15:1 (alpha:beta) in the dynactin complex."

"Both isoforms were found predominantly in the cytosolic fraction as a part of a previously identified 20S complex (referred to as the dynactin complex) with no evidence for a free pool of either isoform."

"Together with CapZαβ binding to the barbed end this results in a highly stable complex of an exactly defined length."

"They bind adjacent clefts between Arp1-D & F (chain-1) and Arp1-F & β-actin-H (chain-2) (Fig. 5F,G)."

"Both consist of four subdomains surrounding a nucleotide binding site (fig. S4)."

"Only Arp11 directly caps the pointed end, suggesting that the other components have a different role such as cargo attachment (28)."

"we purified dynactin from human cell culture and solved the first cryo-EM structure of human dynactin"

"these results suggest that Dre1 binds to the pointed-end complex of dynactin"

"Arp1 was found to polymerize rapidly into short filaments that were similar, but not identical, in length to those in dynactin."

"on the basis of conserved sequence features, is predicted to bind ATP and possibly polymerize"

"Antibodies to the actin-related protein Arp1 (previously referred to as actin-RPV), bound at various sites along the filament, demonstrating that this protein assembles in a polymer similar to conventional actin."

"Alignment of the Arp11 sequence with actin reveals overall conservation of the “actin fold,” a primordial core structure that contains nucleotide and metal binding elements"

"Cryo-electron microscopy (cryo-EM) showed how their coiled-coils run along dynactin’s filament, positioning dynein in a conformation that relieves its intrinsic inhibition"

"These subunits transiently colocalized with dynein foci at the cell cortex and were essential for the cortical anchoring of dynein."

"We have cloned one of the genes, nudK, and determined that it encodes the actin-related protein Arp1, which is a component of the dynactin complex. This provides the first evidence that dynactin is involved in nuclear migration in A. nidulans."

"Consistent with the presence of a true dynactin complex in yeast, Nip100p exists in a stable complex with Act5p as well as Jnm1p, another protein required for proper spindle partitioning during anaphase."

"RNA interference studies revealed an important role for ACTR1A in induction of pro-inflammatory cytokines."

"In this study, we report the identification of α-centractin (ACTR1A) as a novel SETD3 substrate in vitro."

"The filament contains 8 copies of ACTR1A and 1 ACTB."

"DR PAN-GO; P61163; 2 GO annotations based on evolutionary models."

"| ACTR10 | 28.0 | 25 | 10 | 40.0 |"

"ACTR1A chains with a modelled nucleotide: **8/8**, ligand(s) ADP."

"Positions present in **every** large interface: 44, 45, 46, 47, 48, 49, 51, 52, 65, 66, 67, 68, 205, 213, 243, 244, 245, 246. In beta-actin numbering, those below residue 70 (subdomain 2) are [40, 41, 42, 43, 44, 45, 47, 48, 61, 62, 63, 64] and the rest are [200, 208, 242, 243, 244, 245]."