file:PSEPK/PP_2928/PP_2928-uniprot.txt

UniProtKB entry for Pseudomonas putida KT2440 PP_2928

UniProt records Q88IS1 in the CASDH-related PTHR43796 family but retains a saccharopine-dehydrogenase product name.

file:projects/P_PUTIDA/deep-research/PSEPK__bacterial-carboxyspermidine-biosynthesis__ppu00330-deep-research-openscientist.md

OpenScientist PSEPK carboxyspermidine pathway review

The species-aware review identifies PP_2928 as the missing CASDH using family, synteny, and protein-length evidence.

PMID:19196710

An alternative polyamine biosynthetic pathway is widespread in bacteria and essential for biofilm formation in Vibrio cholerae.

The study experimentally identifies paired CANSDH/CANSDC enzymes in the alternative bacterial polyamine pathway.

"deletion of either CANSDC or the putative CANSDH led to loss of sym-norspermidine and spermidine"

PMID:36283333

Structural analysis of carboxyspermidine dehydrogenase from Helicobacter pylori.

The study defines NADPH-dependent condensation of putrescine and aspartate semialdehyde by CASDH.

"CASDH condenses putrescine and aspartate β-semialdehyde into carboxyspermidine in an NADPH-dependent manner."

file:PSEPK/PP_2928/PP_2928-notes.md

Manual evidence notes for PP_2928

The notes record the family and synteny evidence without relying on unreproducible identity values.

file:PSEPK/PP_2928/PP_2928-deep-research-openscientist.md

OpenScientist functional review of PP_2928

The completed retrieval supports CASDH assignment from family, synteny, pathway completeness, and comparative literature.