Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
Pathway for lipid A biosynthesis in Arabidopsis thaliana resembling that of Escherichia coli.
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RNAi against all five tandem AtLpxC genes lowers lipid X to ~37% of wild type and suppresses lipid X accumulation in atlpxb-1, placing LpxC upstream of LpxB.
"The lipid X level decreased in AtLpxC-RNAi transgenic plants to approximately 37% of the amount present in the Col-0 wild type"
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Which of the AtLpxC copies is functional in vivo was not resolved.
"However, it remains unclear which of the closely related AtLpxC enzymes is functional in vivo"
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AtLpxC1.1-GFP (At1g24793 product) is mitochondrial and not chloroplastic.
"The GFP fluorescence from the full-length GFP fusions of AtLpxA and AtLpxC1.1 localized in mitochondria"
Identification of cleavage sites and substrate proteins for two mitochondrial intermediate peptidases in Arabidopsis thaliana.
Detecting Gene Ontology misannotations using taxon-specific rate ratio comparisons.
LPXC1-5 paralog identity and active-site conservation versus E. coli LpxC
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The five canonical AtLpxC products are 100% identical; each conserves the E. coli LpxC Zn(2+) ligands, D246 and the proton donor H265.
"conserved: H79 -> H109, H238 -> H281, D242 -> D285, D246 -> D289, H265 -> H313."
UniProt entry P0DKB7 (LPXC2_ARATH)