Gstt1 review notes

Evidence summary

• [UniProtKB:Q01579] UniProt describes GSTT1 as a glutathione-conjugating enzyme with dichloromethane dehalogenase activity.
• PMID:20097269 The fetched GOA file uses this publication for glutathione transferase activity and glutathione metabolic process.
• PMID:11453994 The fetched GOA file uses this publication for alkylhalidase activity and dichloromethane metabolic process.

Curation decisions

• Core function: glutathione S-transferase theta-1 (glutathione transferase activity, GO:0004364).
• Specific catalytic activities and direct metabolic processes were accepted.
• Broad parent, localization, binding, and stimulus-response annotations were modified, kept non-core, or marked over-annotated according to support.

Re-review 2026-10-04

GOA changes. Four new seeded rows, all donor splits: glutathione transferase activity (GO:0004364), cytosol (GO:0005829) and glutathione metabolic process (GO:0006749, involved_in) from human GSTT1 (UniProtKB:P30711), and glutathione metabolic process (involved_in) from mouse Gstt1 (MGI:MGI:107379, UniProt Q64471), which sits beside the existing acts_upstream_of_or_within row from the same donor. No retired rows. Total 29 rows.

Actions.
- GO:0004364 ISO (human GSTT1): PENDING -> ACCEPT, matching the mouse-donor row and the rat IDA rows (PMID:20097269, PMID:12588193).
- GO:0005829 ISO (human GSTT1): PENDING -> KEEP_AS_NON_CORE, as for the mouse-donor row; rat enzyme purified from liver cytosol.
- GO:0006749 ISO involved_in (mouse Gstt1; human GSTT1): PENDING -> ACCEPT, consistent with IBA/IDA rows.
- No action changed on existing rows. The IEA response to salicylic acid row (ARBA) cited a UniProt DR GO line no longer present in the refreshed flat file; it now cites the rat NSAID induction data PMID:9729437 and stays MARK_AS_OVER_ANNOTATED as stimulus-response context. Donors are now named on the existing ISO rows.
- Description rewritten as standalone biology (removed "The review accepts ..." commentary).

Open questions. None new.

Review follow-up 2026-10-17 (PR #4616 review). Finding 2: all twelve DR GO; cross-reference quotes replaced with the relevant UniProt CC line (CATALYTIC ACTIVITY RX+glutathione, SUBCELLULAR LOCATION Cytoplasm for the cytoplasm/cytosol rows); the circular DR GO; entry removed from both GO:0004602 glutathione-peroxidase rows, which retain the Falcon cumene-hydroperoxide peroxidase-activity quote. Validates clean; 0 stale.