Gene Ontology annotation through association of InterPro records with GO terms
Automatic assignment of GO terms using logical inference, based on on inter-ontology links
TreeGrafter-generated GO annotations
Combined Automated Annotation using Multiple IEA Methods
UniProtKB entry for PSEPK proS
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HAMAP MF_01569 and the ProRS-INS editing-domain assignment support alanine proofreading. The curated rule explicitly distinguishes mischarged Cys-tRNA(Pro), which is not edited by this ProRS activity.
"Reaction=tRNA(Pro) + L-proline + ATP = L-prolyl-tRNA(Pro) + AMP +"
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The curated family rule places the enzyme in the cytoplasm.
"SUBCELLULAR LOCATION: Cytoplasm"
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The source specifically assigns deacylation of Ala-tRNA(Pro), so the supported descendant GO:0043906 is preferable to the generic deacylase term. The Ala-AMP pretransfer reaction is separate, and Cys-tRNA(Pro) editing is explicitly excluded by the curated family rule.
"tRNA(Pro). The misacylated Cys-tRNA(Pro) is not edited by ProRS."
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The curated ProRS rule assigns posttransfer removal of alanine-charged tRNA(Pro).
"'posttransfer' editing and involves deacylation of mischarged Ala-"