"The protein we named SAGA2 (Cre09.g394621)"

"We observed that SAGA2 also localized to that interface but appeared to cover the surface of the matrix more homogeneously than SAGA1."

"Rubisco bound to all predicted internal motif sites when we incubated purified Rubisco with arrays of peptides tiling across the full-length proteins"

"SAGA2, RBMP1, RBMP2, and CSP41A by affinity purification–mass spectrometry"

"At the periphery of the matrix, the motif on starch-binding proteins SAGA1 and SAGA2 mediates interactions between the matrix and surrounding starch sheath."

"We observed that both the 10×His-mVenus-2×SAGA1-CBM20 and the 10×His-mVenus-SAGA2-2×CBM20 proteins, but not a 10×His-mVenus control protein, bound to starch"

"SAGA2 natively has two consecutive CBM20 domains, both of which were included in the SAGA2 construct for this experiment"

"Binding of 10×His-mVenus-2×SAGA1-CBM20 and the 10×His-mVenus-SAGA2-2×CBM20 to starch was decreased in the presence of either β-cyclodextrin or maltoheptaose, suggesting that each protein bound to all three molecules."

"Our results indicate that whereas SAGA1 is enriched at the starch–tubule–matrix junctions of the pyrenoid, SAGA2 is depleted from these regions"

"Taken together, these results indicate that SAGA2 is necessary for normal matrix–starch sheath coverage early during pyrenoid formation."

"other factors can compensate for a lack of SAGA2 to assemble an apparently normal starch sheath during later stages of pyrenoid formation"

"SAGA2 is not necessary for growth under limiting CO2 conditions"

"These observations indicate that SAGA2 is not necessary for pyrenoid tubule formation."

"Remarkably, at both time points, the saga1;saga2 mutant completely lacked starch sheaths and had almost no starch associated with its visible pyrenoid condensates"

"suggesting that SAGA1 and SAGA2 function redundantly to localize starch sheaths to the pyrenoid"

"We conclude that in the saga1;saga2 double mutant, starch is distributed throughout the stroma rather than to the pyrenoid matrix condensates."

"SAGA2 is a ~190-kDa protein that is 30% identical to SAGA1 and has four RBMs and a CBM20 domain"

"the native SAGA2 transit peptide could target SAGA2::mNeon to the chloroplast stroma"

"Similar to SAGA1, SAGA2 interacted with both CrRbcS and the Chlamydomonas large subunit of Rubisco (CrRbcL)"

"SAGA2 could not interact with the EPYC1 helix KO, indicating that EPYC1 interacts with SAGA2 through the same α-helices sequences required for interaction with CrRbcS"

"Together, our imaging data suggested that SAGA2 interacts differently with the condensate compared to SAGA1 but is also able to recruit starch to the matrix."

"but it is unlikely to contribute to matrix-traversing membrane biogenesis based on the growth phenotypes of the saga2 mutant"