Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
Glucosylceramide and galactosylceramide, small glycosphingolipids with significant impact on health and disease.
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UGT8/CGT synthesizes GalCer by transferring galactose from UDP-galactose to the C-1 hydroxyl of ceramide on the luminal side of the ER, where it is a type I membrane glycoprotein with its active site facing the ER lumen.
"GalCer is synthesized on the luminal side of the ER, as UGT8 is a type I membrane glycoprotein with its active site directed into the lumen of this organelle"
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UGT8 and glucosylceramide synthase (GCS/UGCG) share no significant sequence homology, indicating the galactosyl- and glucosyl-ceramide routes are of different evolutionary origin.
"the amino acid sequence of UGT8 shows no significant homology to GCS, which indicates different evolutionary origins of these enzymes"
The human gene CGT encoding the UDP-galactose ceramide galactosyl transferase (cerebroside synthase): cloning, characterization, and assignment to human chromosome 4, band q26.
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CGT (UGT8) is the key enzyme in the biosynthesis of cerebrosides and sulfatides, essential constituents of CNS and PNS myelin, and is important in oligodendrocyte lineage differentiation.
"biosynthesis of the glycosphingolipids, cerebrosides and sulfatides, essential"
UGT8 transfers Gal from UDP-Gal to CERA
Glycosphingolipid biosynthesis