The Pseudomonas aeruginosa liuE gene encodes the 3-hydroxy-3-methylglutaryl coenzyme A lyase, involved in leucine and acyclic terpene catabolism.
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A liuE mutant cannot use leucine/isovalerate or acyclic terpenes as carbon source.
"A mutant in the liuE gene (ORF PA2011) of P. aeruginosa was unable to utilize both leucine/isovalerate and acyclic terpenes as the carbon source."
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Purified recombinant LiuE has HMG-CoA lyase activity with measured kinetics.
"LiuE showed HMG-CoA lyase optimal activity at a pH of 7.0 and 37 degrees C, an apparent K(m) of 100 microM for HMG-CoA and a V(max) of 21 micromol min(-1) mg(-1)."
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The gene encodes the HMG-CoA lyase required for growth on both leucine and acyclic terpenes.
"These results demonstrate that the liuE gene of P. aeruginosa encodes for the HMG-CoA lyase, an essential enzyme for growth in both leucine and acyclic terpenes."
The bifunctional role of LiuE from Pseudomonas aeruginosa, displays additionally HIHG-CoA lyase enzymatic activity.
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The purified protein has HIHG-CoA lyase activity in addition to HMG-CoA lyase activity, giving it a role in both the leucine/isovalerate and acyclic terpene pathways.
"The LiuE protein was purified as a His-tagged recombinant protein and in addition to show HMG-CoA lyase activity"
The atu and liu clusters are involved in the catabolic pathways for acyclic monoterpenes and leucine in Pseudomonas aeruginosa.
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The cluster-level study inferred, rather than measured, that liuE encodes a probable bifunctional HMG-CoA lyase essential for both pathways.
"The liuE gene encodes a probable hydroxy-acyl-CoA lyase (probably HMG-CoA lyase), an enzyme with bifunctional activity that is essential for both AMTC and leucine degradation."
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The atu cluster has no HMG-CoA lyase homologue of its own, which is why liuE serves the terpene pathway too.
"it was named the atu cluster (acyclic terpene utilization), consisting of the atuCDEF genes and lacking the hydroxymethyl-glutaryl-coenzyme A (CoA) lyase (HMG-CoA lyase) homolog."
Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Combined Automated Annotation using Multiple IEA Methods