Gene Ontology annotation through association of InterPro records with GO terms

The InterPro-to-GO mapping for RCK domains does not distinguish between pore-forming and regulatory subunits

Automatic assignment of GO terms using logical inference, based on inter-ontology links

Inferred transport process from transporter activity MF term, but the MF annotation itself is incorrect

Bacterial Interactomes: Interacting Protein Partners Share Similar Function and Are Validated in Independent Assays More Frequently Than Previously Reported.

High-throughput AP-MS interactome study in D. vulgaris Hildenborough

"We have identified 459 high confidence PPIs from D. vulgaris and 391 from Escherichia coli"
The study acknowledges limitations of high-throughput interaction screens

"most of which are between functionally unrelated proteins. The accuracy of these networks, however, is under debate"

PMID:38856222

Bacterial cell volume regulation and the importance of cyclic di-AMP

Authoritative 2024 review on c-di-AMP regulation of K+ transport

"cyclic di-AMP is a master regulator of cell volume and that other cellular processes can be connected with cyclic di-AMP through this core function"

PMID:38719864

Structural basis and synergism of ATP and Na+ activation in bacterial K+ uptake system KtrAB

Cryo-EM structures of B. subtilis KtrAB in ATP and ADP-bound states

"Structural basis and synergism of ATP and Na(+) activation in bacterial K(+) uptake system KtrAB"

PMID:36846772

Redundant potassium transporter systems guarantee the survival of Enterococcus faecalis under stress conditions

KtrA-associated systems important for survival under osmotic and pH stress

"The potassium uptake transporters KtrI and KtrII were described in Enterococcus hirae, which were associated with growth in acidic and alkaline conditions"

file:DESVH/Q72F06/Q72F06-deep-research-falcon.md

Deep research summary for Q72F06 (DVU_0412) TrkA-like protein

Q72F06 is a cytosolic RCK regulatory subunit, not a membrane transporter

"Substrate of the overall system: K+; DVU_0412 itself is not the pore but the ligand-gated regulator controlling K+ flux through the membrane partner"
Forms octameric gating ring that docks to membrane channel

"Architecturally, the RCK subunits form an octameric gating ring that docks to the dimeric membrane pore to control K+ flux"
ATP/ADP-dependent gating mechanism

"ATP binding to DVU_0412's RCK domains promotes an active, open-channel state in the associated membrane pore; ADP stabilizes a closed/inactive conformation"
Cytosolic localization

"Cytosolic oligomeric ring apposed to the inner membrane, docking to a TrkH/KtrB pore"
Role in potassium homeostasis

"Potassium uptake and cell volume control; functionally redundant with other K+ systems typical of bacteria, enabling stress resilience"