file:interpro/panther/PTHR12792/PTHR12792-review.md

PANTHER family review PTHR12792: IBA propagation assessment for cut1

Gene Ontology annotation through association of InterPro records with GO terms

Annotation inferences using phylogenetic trees

Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt

GO_REF:0000117

Electronic Gene Ontology annotations created by ARBA machine learning models

GO_REF:0000120

Combined Automated Annotation using Multiple IEA Methods

PMID:12390246

Cut1/separase C-terminus affects spindle pole body positioning in interphase of fission yeast: pointed nuclear formation.

Separase activated by securin destruction cleaves the cohesin subunit Scc1/Rad21 enriched in kinetochores; Cut1 resides in interphase cytoplasm and mobilizes to the spindle and SPBs in mitosis. A C-terminal, protease-independent activity affects SPB positioning (pointed nuclear formation).

PMID:14532136

Rec8 cleavage by separase is required for meiotic nuclear divisions in fission yeast.

Separase Cut1 cleaves the meiotic kleisin Rec8 at two identified sites; a non-cleavable Rec8 blocks homologue disjunction at meiosis I, establishing Cut1's meiotic endopeptidase function.

PMID:15329725

Separase-mediated cleavage of cohesin at interphase is required for DNA repair.

The securin-separase (Cut2-Cut1) complex aids DNA repair by cleaving cohesin locally in interphase; the function requires separase protease activity and cleavable cohesin.

PMID:16823372

ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.

Genome-wide YFP localization assigns Cut1 to the cytosol.

PMID:1934126

Common genes and pathways in the regulation of the mitotic and meiotic cell cycles of Schizosaccharomyces pombe.

A cut1 cut2 double mutant forms two-spored asci whereas single cut1 or cut2 mutants do not affect sporulation, indicating involvement in the meiotic program.

PMID:8065367

Identification of cut8+ and cek1+, a novel protein kinase gene, which complement a fission yeast mutation that blocks anaphase.

cut1+ is a multicopy suppressor of the anaphase-blocking cut8-563 mutation and is described as essential for nuclear division.

PMID:8978688

Fission yeast Cut1 and Cut2 are essential for sister chromatid separation, concentrate along the metaphase spindle and form large complexes.

cut1 ts mutants fail to separate sister chromatids in anaphase (cut phenotype); Cut1 concentrates along the metaphase spindle and forms a large complex with securin Cut2; Cut2 is degraded in anaphase while Cut1 is stable throughout the cell cycle.

PMID:9635190

Cut1 is loaded onto the spindle by binding to Cut2 and promotes anaphase spindle movement upon Cut2 proteolysis.

Cut1 is cytoplasmic in interphase and relocates to SPBs and the spindle at prophase; Cut2 loads Cut1 onto the spindle, and Cut2 proteolysis is needed for active Cut1 participation in sister chromatid separation and anaphase spindle movement.