Gene Ontology annotation through association of InterPro records with GO terms
Gene Ontology annotation based on Enzyme Commission mapping
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Automatic assignment of GO terms using logical inference, based on on inter-ontology links
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
Drosophila Topors is a RING finger-containing protein that functions as a ubiquitin-protein isopeptide ligase for the hairy basic helix-loop-helix repressor protein.
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This PMID appears unrelated to ARL8B chromosome segregation and should be checked as a possible upstream GOA/UniProt reference error.
"Drosophila Topors is a RING finger-containing protein that functions as a ubiquitin-protein isopeptide ligase for the hairy basic helix-loop-helix repressor protein."
Novel small GTPase subfamily capable of associating with tubulin is required for chromosome segregation.
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ARL8B/GIE is a small GTPase with tubulin binding and mitotic spindle-midzone localization.
"Gie protein has ability to bind to tubulin and localizes with microtubules on the spindle mid-zone in late mitosis."
An N-terminally acetylated Arf-like GTPase is localised to lysosomes and affects their motility.
The Arf-family protein, Arl8b, is involved in the spatial distribution of lysosomes.
Large-scale proteomics and phosphoproteomics of urinary exosomes.
Defining the membrane proteome of NK cells.
Lysosomal trafficking, antigen presentation, and microbial killing are controlled by the Arf-like GTPase Arl8b.
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ARL8B is required for lysosomal cargo delivery, CD1 antigen presentation, and phagosome-lysosome fusion through VPS41/HOPS recruitment.
"Arl8b silencing resulted in a delay in delivery of fluid phase dextran, receptor-internalized low-density lipoprotein (LDL), and CD1 cargo to lysosomes."
Arl8 and SKIP act together to link lysosomes to kinesin-1.
Arf-like GTPase Arl8b regulates lytic granule polarization and natural killer cell-mediated cytotoxicity.
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ARL8B promotes lytic granule and MTOC polarization required for NK-cell cytotoxicity.
"Arl8b drives the polarization of lytic granules and microtubule-organizing centers (MTOCs) toward the immune synapse"
BORC, a multisubunit complex that regulates lysosome positioning.
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BORC recruits ARL8B to lysosomes to promote microtubule-guided peripheral lysosome transport.
"BORC functions to recruit Arl8 to lysosomes, thereby initiating a chain of interactions that promotes microtubule-guided transport of lysosomes toward the cell periphery."
RNF167 targets Arl8B for degradation to regulate lysosome positioning and endocytic trafficking.
The Rab7 effector PLEKHM1 binds Arl8b to promote cargo traffic to lysosomes.
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PLEKHM1 connects Rab7 and ARL8B to recruit HOPS and promote lysosomal degradation of endocytic and autophagic cargo.
"Arl8b binding is crucial for PLEKHM1 to promote lysosomal degradation of endocytic and autophagic cargo."
Lysosome-Mediated Plasma Membrane Repair Is Dependent on the Small GTPase Arl8b and Determines Cell Death Type in Mycobacterium tuberculosis Infection.
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ARL8B-dependent lysosome exocytosis supports plasma membrane repair during Mycobacterium infection.
"Arl8b is required for the process of plasma membrane repair in controlling the Ca2+-dependent exocytosis of lysosomes"
A reference map of the human binary protein interactome.
β-Coronaviruses Use Lysosomes for Egress Instead of the Biosynthetic Secretory Pathway.
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Beta-coronaviruses use an ARL8B-dependent lysosomal exocytic egress pathway.
"these viruses use a lysosomal, Arl8b-dependent exocytic pathway for release into the extracellular environment."
RUFY3 and RUFY4 are ARL8 effectors that promote coupling of endolysosomes to dynein-dynactin.
RUFY1 binds Arl8b and mediates endosome-to-TGN CI-M6PR retrieval for cargo sorting to lysosomes.
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ARL8B regulates RUFY1 endosomal localization and CI-M6PR retrieval from endosomes to the TGN.
"RUFY1 depletion led to a delay in CI-M6PR retrieval from endosomes to the TGN, resulting in impaired delivery of newly synthesized hydrolases to lysosomes."
DENND6A links Arl8b to a Rab34/RILP/dynein complex, regulating lysosomal positioning and autophagy.
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DENND6A is a direct ARL8B effector recruited to peripheral lysosomes, where it acts as a GEF for Rab34; activated Rab34 recruits a RILP/dynein complex to drive retrograde lysosome transport and nutrient-dependent juxtanuclear repositioning, and loss of DENND6A impairs autophagic flux. This positions ARL8B as a bidirectional transport coordinator, distinct from the RUFY3/RUFY4 dynein-dynactin coupling.
Biallelic BORCS8 variants cause an infantile-onset neurodegenerative disorder with altered lysosome dynamics.
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Biallelic loss-of-function variants in the BORC subunit BORCS8 cause an infantile-onset neurodegenerative disorder; BORC sequentially recruits ARL8 and kinesin motors to lysosomes, and variant alleles reduce BORC assembly and peripheral lysosome distribution. This is human disease evidence for the upstream BORC-to-ARL8B anterograde axis, but concerns BORCS8 rather than an ARL8B coding mutation.
UniProtKB reviewed entry for human ARL8B (Q9NVJ2)
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Reviewed UniProt synthesis places ARL8B on lysosomal/endolysosomal membranes as a small GTPase controlling lysosomal positioning, transport, fusion, and exocytosis.
"Localizes specifically to lysosomal membranes and mediates anterograde lysosomal motility"
Proteostasis PN projected candidate additions for ARL8B
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The PN projection suggests ARL8B to GO:0061906 autophagosome localization, but the gene-specific review treats this conservatively because direct evidence is stronger for lysosome localization and autophagosome-lysosome fusion.
"ARL8B GO:0061906 autophagosome localization new_to_goa"