GFI1B recognizes regulatory DNA and recruits repressive transcriptional complexes. One core describes this mechanism and its roles in erythrocyte and megakaryocyte differentiation. Promoter-dependent activation and the measured CPAP association remain separate contexts. The p37 and p32 functional classes map to the two verified natural sequences; the unidentified p39 band and engineered fragments are not additional products.
The manual evidence page links the original FOG1 experiment, GATA1 recruitment assays, SELEX workbook and exact interaction records. Human GFI1B–GATA1 experiments support transcription-factor binding. The incoming FOG1 identifier still needs a source check: the cited FOG1 immunoprecipitation was negative in its tested mouse setting. Accepting the molecular function does not validate that partner field. The negative-G1/S term remains a separate directional source query.
CPAP binding has both Y2H and mammalian pull-down support and fits the partner-class definition. It does not establish a physiological cytoskeletal role for GFI1B. NELL2 and JAG2 associations lack the additional signaling role required by receptor or ligand activity. All three positive pairs remain documented.
Thirteen compact excerpts make the principal judgments checkable. The nuclear-matrix sentence and ETO sentence together use 22 words from PMID:12874834. The FOG1 excerpt comes from separately accessed original text and appears in both the manual page and the annotation; its abstract-only cache is not represented as the source of that quote. Official UniProt excerpts retain their precise FUNCTION fields and evidence arrays. The SELEX index is a derived workbook locator, not a sentence from the paper.
The source evidence archive preserves all 21 incoming records, dates and IDs, the genuine seed and the 23 source objects. It also distinguishes the asserted PTHR24393 ancestry from current PTHR23226 metadata. Source access notes separate inspected original sections from abstract-only assessments. PMID:9878267 has verified identifier metadata but unverified biological content. Acquisition, validation and amendment provenance are recorded in the archive and session history.
The amendment passed default validation, strict matching of all 21 original GOA contexts, computed-status confirmation, genuine EDIT history validation and private rendering. The first check reported missing propagation metadata; the added object records the valid AN29/PTN008616677 inheritance and its human process refinement. Final status is COMPLETE with zero annotation advisories. Earlier CREATE-session outcomes remain historical; renewed substantive review and ordinary CI are still required before readiness.