Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Combined Automated Annotation using Multiple IEA Methods
Human cytosolic 5'-nucleotidase I: characterization and role in nucleoside analog resistance.
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Cloned and characterized the human cytosolic 5'-nucleotidase cN-I; recombinant enzyme shows high affinity toward dCMP and lower affinity toward AMP and IMP, and requires ADP for maximal activity.
"The recombinant cN-I showed high affinity toward dCMP and"
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Expressed at a high level in skeletal and heart muscle; implicated in pyrimidine nucleotide pool regulation and nucleoside-analog resistance.
"It is expressed at a high level in skeletal and heart muscle,"
A proteome-scale map of the human interactome network.
Extensive disruption of protein interactions by genetic variants across the allele frequency spectrum in human populations.
A reference map of the human binary protein interactome.
The decreased serum activity of cytosolic 5'-nucleotidase IA as a potential marker of breast cancer-associated muscle inflammation.
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cN-IA plays a central role in regulating the skeletal-muscle purine nucleotide pool, preferentially converting AMP to adenosine, and acts as an autoantigen in muscle diseases.
"preferentially converting adenosine monophosphate to adenosine."
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cN-IA can act as an autoantigen in inflammatory muscle disease.
"cN-IA can act as an autoantigen in muscle"
Regulation of the fatty acid composition of alkyl ether phospholipid in Ehrlich ascites tumor cells. The substrate specificities of 1-O-alkylglycerol 3-phosphate and 1-O-alkylglycero-3-phosphocholine acyltransferases.
Kinetics of adenylate metabolism in human and rat myocardium.
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Assayed AMP- and IMP-specific 5'-nucleotidases (and other purine degradation/salvage enzymes) in human and rat heart cytoplasm as part of the adenosine-producing pathway.
"AMP- and IMP-specific 5'-nucleotidases, adenosine"
Soluble forms of 5'-nucleotidase in rat and human heart.
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Measured cytosolic AMP-preferring (N-I) 5'-nucleotidase activity in human left ventricle; showed cytosolic 5'-nucleotidase can account for adenosine formation during cardiac ischemia; Mg2+-dependent, pH optimum ~7.0.
"5'-nucleotidase activity in human heart can easily account for adenosine"
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Human heart cytosolic AMP-preferring 5'-nucleotidase (N-I) activity was quantified.
"the AMP-preferring one (N-I, 107 +/- 61 mU/g,"
(d)CMP, TMP, or (d)UMP + H2O => (deoxy)cytidine, thymidine, or (deoxy)uridine + orthophosphate (NT5C1A)
(2'-deoxy)purine nucleoside 5'-monophosphate + H2O => (2'-deoxy)purine nucleoside + orthophosphate [NT5C1A]