Gene Ontology annotation through association of InterPro records with GO terms

Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt

GO_REF:0000120

Combined Automated Annotation using Multiple IEA Methods

file:NICAT/NaAO2_candidate_AO_0/NaAO2_candidate_AO_0-uniprot.txt

UniProt entry A0A1J6KBX2 for Nicotiana attenuata AO_0

AO_0 is annotated as an L-aspartate oxidase in NAD precursor metabolism

"DE RecName: Full=L-aspartate oxidase"
UniProt places AO_0 in chloroplast-localized NAD biosynthetic chemistry

"CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast"

file:NICAT/NaAO2_candidate_AO_0/NaAO2_candidate_AO_0-notes.md

NaAO2 AO_0 candidate notes

AO_0 is a plausible upstream pyridine-branch nicotine candidate

"UniProt curates A0A1J6KBX2 as an L-aspartate oxidase/NadB-family flavoprotein that catalyzes oxidation of L-aspartate to iminoaspartate, linking this candidate to the upstream NAD branch that feeds pyridine alkaloid biosynthesis."
AO_0 versus AO_1 remains an explicit paralog-resolution problem

"AO_0 is therefore a plausible NaAO2 candidate, but the current public record does not distinguish it cleanly from AO_1 as the nicotine-pathway-specialized copy."