Gene Ontology annotation through association of InterPro records with GO terms
Gene Ontology annotation based on Enzyme Commission mapping
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
SHP2-interacting transmembrane adaptor protein (SIT), a novel disulfide-linked dimer regulating human T cell activation.
Tyrosine 319 in the interdomain B of ZAP-70 is a binding site for the Src homology 2 domain of Lck.
Galectin-1, a natural ligand for the receptor-type protein tyrosine phosphatase CD45.
Lad, an adapter protein interacting with the SH2 domain of p56lck, is required for T cell activation.
Self-association of the SET domains of human ALL-1 and of Drosophila TRITHORAX and ASH1 proteins.
The tyrosine kinase Hck is an inhibitor of HIV-1 replication counteracted by the viral vif protein.
Tyrosine residues in phospholipase Cgamma 2 essential for the enzyme function in B-cell signaling.
Phosphorylation-dependent interactions between ADAM15 cytoplasmic domain and Src family protein-tyrosine kinases.
BY55/CD160 acts as a co-receptor in TCR signal transduction of a human circulating cytotoxic effector T lymphocyte subset lacking CD28 expression.
Calpain II colocalizes with detergent-insoluble rafts on human and Jurkat T-cells.
Cutting edge: Fyn is essential for tyrosine phosphorylation of Csk-binding protein/phosphoprotein associated with glycolipid-enriched microdomains in lipid rafts in resting T cells.
The influence of the src-family kinases, Lck and Fyn, on T cell differentiation, survival and activation.
Regulation of Fyn through translocation of activated Lck into lipid rafts.
Src family kinases in tumor progression and metastasis.
Tyrosyl phosphorylation of Shp2 is required for normal ERK activation in response to some, but not all, growth factors.
Steel factor stimulates the tyrosine phosphorylation of the proto-oncogene product, p95vav, in human hemopoietic cells.
Subdomain X of the kinase domain of Lck binds CD45 and facilitates dephosphorylation.
Unc119, a novel activator of Lck/Fyn, is essential for T cell activation.
Molecular basis for a direct interaction between the Syk protein-tyrosine kinase and phosphoinositide 3-kinase.
Characterization of Lck-binding elements in the herpesviral regulatory Tip protein.
The influence of SRC-family tyrosine kinases on Na,K-ATPase activity in lens epithelium.
Profound block in thymocyte development in mice lacking p56lck.
The C terminus of T cell-specific adapter protein (TSAd) is necessary for TSAd-mediated inhibition of Lck activity.
Tyrosine phosphorylation of the Tio oncoprotein is essential for transformation of primary human T cells.
Regulation of Bcl-3 through interaction with the Lck tyrosine kinase.
A rare mRNA variant of the human lymphocyte-specific protein tyrosine kinase LCK gene with intron B retention and exon 7 skipping encodes a putative protein with altered SH3-dependent molecular interactions.
The tyrosine kinase Lck is a positive regulator of the mitochondrial apoptosis pathway by controlling Bak expression.
Inhibition of SRC tyrosine kinases suppresses activation of nuclear factor-kappaB, and serine and tyrosine phosphorylation of IkappaB-alpha in lipopolysaccharide-stimulated raw 264.7 macrophages.
Molecular basis for regulation of Src by the docking protein p130Cas.
T cell activation-induced CrkII binding to the Zap70 protein tyrosine kinase is mediated by Lck-dependent phosphorylation of Zap70 tyrosine 315.
Identification of substrates of human protein-tyrosine phosphatase PTPN22.
Structure function analysis of SH2D2A isoforms expressed in T cells reveals a crucial role for the proline rich region encoded by SH2D2A exon 7.
Glucocorticoids cause rapid dissociation of a T-cell-receptor-associated protein complex containing LCK and FYN.
Magicin associates with the Src-family kinases and is phosphorylated upon CD3 stimulation.
Evidence of LAT as a dual substrate for Lck and Syk in T lymphocytes.
Growth transformation of human T cells by herpesvirus saimiri requires multiple Tip-Lck interaction motifs.
Crystal structure analysis and solution studies of human Lck-SH3; zinc-induced homodimerization competes with the binding of proline-rich motifs.
A proline to glycine mutation in the Lck SH3-domain affects conformational sampling and increases ligand binding affinity.
Carcinoembryonic antigen-related cell adhesion molecule 1 inhibits proximal TCR signaling by targeting ZAP-70.
Distinct interactions between ubiquitin and the SH3 domains involved in immune signaling.
Gab2 is involved in differential phosphoinositide 3-kinase signaling by two splice forms of c-Kit.
Large-scale structural analysis of the classical human protein tyrosine phosphatome.
PKD2 interacts with Lck and regulates NFAT activity in T cells.
SOCS-6 negatively regulates T cell activation through targeting p56lck to proteasomal degradation.
Src kinase phosphorylates RUNX3 at tyrosine residues and localizes the protein in the cytoplasm.
MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis.
Tarp regulates early Chlamydia-induced host cell survival through interactions with the human adaptor protein SHC1.
Evidence that the transforming gene of avian sarcoma virus encodes a protein kinase associated with a phosphoprotein.
Substrate specificity of lymphoid-specific tyrosine phosphatase (Lyp) and identification of Src kinase-associated protein of 55 kDa homolog (SKAP-HOM) as a Lyp substrate.
A motif in the V3 domain of the kinase PKC-θ determines its localization in the immunological synapse and functions in T cells via association with CD28.
DHHC2 is a protein S-acyltransferase for Lck.
T cell protein tyrosine phosphatase attenuates T cell signaling to maintain tolerance in mice.
Quantitative analysis of HSP90-client interactions reveals principles of substrate recognition.
GRB2-mediated recruitment of THEMIS to LAT is essential for thymocyte development.
In vitro membrane reconstitution of the T-cell receptor proximal signaling network.
The CD4 and CD8 antigens are coupled to a protein-tyrosine kinase (p56lck) that phosphorylates the CD3 complex.
The phosphatase JKAP/DUSP22 inhibits T-cell receptor signalling and autoimmunity by inactivating Lck.
Enhanced prediction of Src homology 2 (SH2) domain binding potentials using a fluorescence polarization-derived c-Met, c-Kit, ErbB, and androgen receptor interactome.
Identification of SH3 domain proteins interacting with the cytoplasmic tail of the a disintegrin and metalloprotease 10 (ADAM10).
A proteome-scale map of the human interactome network.
Preferred SH3 domain partners of ADAM metalloproteases include shared and ADAM-specific SH3 interactions.
Widespread Expansion of Protein Interaction Capabilities by Alternative Splicing.
ARAP, a Novel Adaptor Protein, Is Required for TCR Signaling and Integrin-Mediated Adhesion.
WNK1 kinase balances T cell adhesion versus migration in vivo.
ABL2 suppresses FLT3-ITD-induced cell proliferation through negative regulation of AKT signaling.
Extensive rewiring of the EGFR network in colorectal cancer cells expressing transforming levels of KRAS(G13D).
A central chaperone-like role for 14-3-3 proteins in human cells.
Galectin-9 promotes natural killer cells activity via interaction with CD44.
Signaling via a CD27-TRAF2-SHP-1 axis during naive T cell activation promotes memory-associated gene regulatory networks.
ITPRIPL1 binds CD3ε to impede T cell activation and enable tumor immune evasion.
Proximity between LAG-3 and the T cell receptor guides suppression of T cell activation and autoimmunity.
Interaction of p56lck with CD4 in the yeast two-hybrid system.
The SH3 domain of p56lck is involved in binding to phosphatidylinositol 3'-kinase from T lymphocytes.
Tyrosine phosphorylation of CD45 phosphotyrosine phosphatase by p50csk kinase creates a binding site for p56lck tyrosine kinase and activates the phosphatase.
Distinct intracellular localization of Lck and Fyn protein tyrosine kinases in human T lymphocytes.
p70 phosphorylation and binding to p56lck is an early event in interleukin-2-induced onset of cell cycle progression in T-lymphocytes.
Lck-dependent tyrosyl phosphorylation of the phosphotyrosine phosphatase SH-PTP1 in murine T cells.
Physical and functional association of p56lck with Fc gamma RIIIA (CD16) in natural killer cells.
Phosphorylation of Ser-42 and Ser-59 in the N-terminal region of the tyrosine kinase p56lck.
Demonstration of a direct interaction between p56lck and the cytoplasmic domain of CD45 in vitro.
Phosphotyrosine-independent binding of a 62-kDa protein to the src homology 2 (SH2) domain of p56lck and its regulation by phosphorylation of Ser-59 in the lck unique N-terminal region.
Molecular cloning of a phosphotyrosine-independent ligand of the p56lck SH2 domain.
Phosphorylated T cell receptor zeta-chain and ZAP70 tandem SH2 domains form a 1:3 complex in vitro.
Human immunodeficiency virus type 1 Nef binds directly to Lck and mitogen-activated protein kinase, inhibiting kinase activity.
Identification of the site in the Syk protein tyrosine kinase that binds the SH2 domain of Lck.
The kinase, SH3, and SH2 domains of Lck play critical roles in T-cell activation after ZAP-70 membrane localization.
Interaction between Sam68 and Src family tyrosine kinases, Fyn and Lck, in T cell receptor signaling.
Tap: a novel cellular protein that interacts with tip of herpesvirus saimiri and induces lymphocyte aggregation.
Human homologue of the Drosophila discs large tumor suppressor binds to p56lck tyrosine kinase and Shaker type Kv1.3 potassium channel in T lymphocytes.
Kit signaling through PI 3-kinase and Src kinase pathways: an essential role for Rac1 and JNK activation in mast cell proliferation.
GRB2 is indirectly recruited to p-KIT through SHP2
Phosphorylation of GAB2 by SFKs
Phosphorylation of SHP2 by SFKs
Synthesis of PIP3 from PIP2 by PI3K
Phosphorylation and activation of VAV1
Disassociation and translocation of STATs to the nucleus
Phosphorylation of p-KIT on Y900 by Src kinases
Indirect recruitment of PI3K to KIT via p(Y)-GAB2
Nef mediated disruption of CD4:Lck Complex
Nef Binds and activates the Src-family tyrosine kinase Lck
Phosphorylation of ITAM motifs in CD3 complexes
Phosphorylation of ZAP-70 by Lck
Dephosphorylation of Lck-pY505 by CD45
Phosphorylation of SLP-76
Inactivation of Lck by Csk
Phosphorylation of TBSMs in LAT
Phosphorylation of PLC-gamma1
Change of PKC theta conformation
Recruitment of ZAP-70 to phosphorylated ITAMs
Cell surface interactions at the vascular wall
Indirect recruitment of GAB2 to p-KIT
SHP2 interacts with p-KIT
Recruitment of CBL to KIT
Interaction of APS and p-KIT
Recruitment of SYK to p-DAP12
Phosphorylation of PECAM-1 by Fyn or Lyn or c-Src
PI3K phosphorylates PIP2 to PIP3
Phosphorylation of LAT by p-SYK
Phosphorylation of SLP-76 by p-SYK
PI3K inhibitors block PI3K catalytic activity
PI3K phosphorylates PIP2 to PIP3
p85 regulatory unit of PI3K binds p-6Y-SYK
Phosphorylation of BTK by p-SYK
Phosphorylation and activation of VAV2/VAV3 by SYK
Phosphorylation of PLC-gamma by p-BTK/p-SYK
SHP2 phosphatase binds CTLA-4
Phosphorylation of CTLA-4
Regulation of T cell activation by CD28 family
CD28 bound PI3K phosphorylates PIP2 to PIP3
Activation of Rac1 by pVav1
Activation of Cdc42 by pVav1
Translocation of Vav1 to CD28
Dephosphorylation of CD3-zeta by PD-1 bound phosphatases
Interaction of SHP-1 or SHP-2 with phospho PD-1
Syk/Lck phosphorylate LAT
Platelet activation, signaling and aggregation
Inactivation of LCK by PTPN22
PTPN22 dephosphorylates ZAP70
RHOH binds effectors at the plasma membrane
Phosphorylation of STATs downstream of KIT mutants
JAK2 binds to p-KIT mutants
Recruitment of STATs by KIT mutants
Dimerization of STATs downstream of KIT mutants
Phosphorylation of JAK2 downstream of KIT mutants
Disassociation and translocation of STATs to the nucleus downstream of KIT mutants
Lck bound to coreceptor is less active than free Lck.
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Coreceptor-free LCK has higher mobility, more activation-loop Y394 phosphorylation and higher kinase activity than CD8alpha-bound LCK (mouse OT-I T cell hybridoma).
"Our data show that free Lck has higher mobility, more Y394 phosphorylation, and higher kinase activity than the coreceptor-bound Lck."
A partial human LCK defect causes a T cell immunodeficiency with intestinal inflammation.
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Homozygous LCK P440S in two siblings causes T cell lymphopenia, recurrent infections and protracted diarrhea; the mouse knock-in spares some conventional T cells but not regulatory T cells, leading to intestinal inflammation.
"These findings demonstrate that P440S LCK spares sufficient T cell function to allow the maturation of some conventional T cells but not regulatory T cells-leading to intestinal inflammation."
Molecular determinants of differential substrate selection between the Src family kinases Lck and Src.
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Substituting the Src kinase domain with that of Lck is sufficient to confer TCR ITAM phosphorylation in T cells, whereas swapping the adaptor (SH3/SH2) domains has minimal effect.
"Domain-swapping experiments further revealed that substitution of the Src kinase domain with that of Lck was sufficient to confer ITAM phosphorylation and trigger downstream TCR signaling responses, whereas exchange of adaptor domains had minimal effect."
CD28-CAR-T cell activation through FYN kinase signaling rather than LCK enhances therapeutic performance.
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CD28-containing CAR signaling can proceed in the absence of LCK via FYN.
"Here, we show that CAR signaling occurs in the absence of LCK."
Falcon deep research report for human LCK