Annotation inferences using phylogenetic trees
Electronic Gene Ontology annotations created by ARBA machine learning models
Spatiotemporal regulation of cell-cycle genes by SHORTROOT links patterning and growth.
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SHR and SCR directly activate CYCD6;1 at the formative division.
"Coincident with the onset of a specific formative division, SHR and SCR directly activate a D-type cyclin"
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SHR and SCR bind the CYCD6;1 promoter (ChIP-qPCR).
"We found significant enrichment for both SHR and SCR binding around 1 kilo-base (kb) upstream of the CYCD6;1 promoter"
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cycd6;1 mutants have fewer CEI-daughter periclinal divisions.
"cycd6;1 CEI-daughter cells showed significantly fewer periclinal divisions than wild type at both early heart and torpedo embryo stages"
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CYCD6;1 is specific to formative, not proliferative, divisions.
"These results indicate that CYCD6;1 is not required for proliferative cell divisions but is specifically involved in formative divisions needed for proper ground tissue patterning"
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Ectopic CYCD6;1 partially rescues shr formative divisions.
"Both CYCD6;1 and CDKB2;1 were able to partially complement the shr formative division phenotype"
A bistable circuit involving SCARECROW-RETINOBLASTOMA integrates cues to inform asymmetric stem cell division.
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CYCD6;1-CDKB1 complexes phosphorylate RBR.
"Based on our genetic and biochemical data, RBR phosphorylation is carried out by a complex of CYCD6;1 with either CDKB1;1 or CDKB1;2"
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CYCD6;1 binding increases CDKB1 activity.
"revealed that CYCD6;1 binding increased basal activity of CDKB1"
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CYCD6;1-YFP is nuclear in some endodermal cells, cytoplasmic in others.
"fluorescence was detected in the nucleus of some endodermis cells and in the cytoplasm of others"
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Auxin induction of CYCD6;1 requires SHR and SCR.
"We concluded that auxin-mediated induction of the CYCD6;1 promoter requires SHR and SCR activity"
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Nested feed-forward loops with SCR-RBR position the asymmetric divisions.
"We demonstrate that this interaction, together with the activity of the RBR regulator CYCD6;1, defines the precise position of ACDs in the stem cell area through two nested feedforward loops"
Targeted interactomics reveals a complex core cell cycle machinery in Arabidopsis thaliana.
Arabidopsis PCNAs form complexes with selected D-type cyclins.
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CycD6;1 forms nuclear complexes with PCNA (BiFC/FRET).
"presence of GFP fluorescence was observed only in the nucleus when the formation of complexes between PCNA1/2 and CycD4;1, CycD5;1, CycD6;1, or CycD7;1 was tested"
NAC1 regulates root ground tissue maturation by coordinating with the SCR/SHR-CYCD6;1 module in Arabidopsis.
Arabidopsis BIRD Zinc Finger Proteins Jointly Stabilize Tissue Boundaries by Confining the Cell Fate Regulator SHORT-ROOT and Contributing to Fate Specification.
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BIRDs regulate SHR targets SCR and CYCD6.
"constraining SHORT-ROOT spread through nuclear retention and transcriptional regulation of key downstream SHORT-ROOT targets, including SCARECROW and CYCLIND6"