Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Electronic Gene Ontology annotations created by ARBA machine learning models
Crystal structure of the mitochondrial chaperone TIM9.10 reveals a six-bladed alpha-propeller
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Crystal structure of Tim9-Tim10 hexamer reveals six-bladed alpha-propeller topology
"The crystal structure of TIM9.10 reveals a previously undescribed alpha-propeller topology in which helical "blades" radiate from a narrow central pore lined with polar residues."
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Twin CX3C motif forms two intramolecular disulfides in each subunit
"In each TIM9.10 subunit, a signature "twin CX3C" motif forms two intramolecular disulfides."
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Tentacle-like blades engage hydrophobic precursor proteins
"The propeller blades are reminiscent of "tentacles" in chaperones Skp and prefoldin."
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Complex functions as chaperone for metabolite carrier precursors in IMS
"They coassemble into a hexamer, TIM9.10, which captures and chaperones precursors of inner membrane metabolite carriers as they exit the TOM channel in the outer membrane."
Organization and function of the small Tim complexes acting along the import pathway of metabolite carriers into mammalian mitochondria
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Tim9-Tim10 forms a 70-kDa hetero-oligomeric complex in the IMS
"A 70-kDa complex that contains Tim9 and Tim10a and a Tim9-10a-10b that is part of a higher molecular weight assembly of 450 kDa."
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Small Tim complexes directly interact with carrier proteins in transit
"During import, the small Tim complexes directly interact with human adenine nucleotide translocator (ANT) in transit in a metal-dependent manner."
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Small Tim proteins interact with Tim22 for carrier insertion
"For insertion of carrier preproteins into the inner membrane, the human small Tim proteins directly interact with human Tim22, the putative insertion pore of the TIM22 translocase."
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Only a small fraction stably associates with Tim22
"However, in contrast to yeast, only a small fraction of Tim9-Tim10a-Tim10b complex is in a stable association with Tim22."
The human family of Deafness/Dystonia peptide (DDP) related mitochondrial import proteins
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DDP/Tim family proteins are zinc-binding proteins involved in carrier import
"It bears a strong resemblance to a recently characterized set of zinc-binding yeast proteins (Tim8p, Tim9p, Tim10p, Tim12p, and Tim13p) that are implicated in the import of a class of transmembrane carrier proteins from the cytoplasm to the mitochondrial inner membrane."
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Establishes orthologous relationships between human and yeast small Tims
"We describe here the human complement of DDP/Tim-like proteins and establish the likely orthologous relationships between sequences from human, yeast, and other organisms."
Deep research review of S. pombe Tim10
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Tim10 forms heterohexameric complex with Tim9 in the IMS
"[Tim10 forms a heterohexamer with Tim9 in the IMS, functioning as an ATP-independent chaperone - inferred from conserved small Tim biology across eukaryotes]"
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Function inferred from conserved small Tim biology
"[No S. pombe-specific experimental papers on tim10 were identified; function is inferred from conserved small Tim biology established in fungi and metazoans]"