PA3170 (MTA deaminase, Q9HZ64) review notes

Review session 2026-11-05 (PseudoCAP IBA-seed protocol, tier 2)

Fetched with just fetch-gene PSEAE Q9HZ64 --alias PA3170; accession matches the worklist. No
deep-research provider used. Both papers cached with full text.

Literature

PMID:23050701
MTA kcat 24.6 per s, Km 1.5 uM; adenosine kcat/Km 3.7 x 10^5 per M per s (40-fold lower); inactive
on adenine, SAH, guanosine. Only MTA-acting activity in extracts; crystal structure with MTCF.
PMID:23968233 (Pa3170 is in sg-2.)
PAO1 has a separate adenosine deaminase, PA0148 (listed among the candidate deaminases in
PMID:23050701).

Annotation decisions

GO:0090614 (IDA): ACCEPT, core.
GO:0004000 (IDA, PseudoCAP seed): KEEP_AS_NON_CORE; genuine secondary activity.
GO:0019239 (IBA, IEA), GO:0016787, GO:0016810, GO:0016814 (IEA): TOO_GENERAL (GO:0090614 is_a all of them).
Action counts: ACCEPT 1, KEEP_AS_NON_CORE 1, TOO_GENERAL 5. No source defects.

PseudoCAP IBA-seed assessment

PTN node	node term	n_seeds	leaf IBAs	seed annotation (term, evidence, PMID)	verdict	rationale	other seeds	IBD stands without this seed?
PTN005182504	GO:0019239 deaminase activity	5	82 (64 taxa)	GO:0004000 adenosine deaminase activity, IDA, PMID:23050701 (PseudoCAP); also GO:0090614 IDA PMID:23968233 (CACAO)	SOUND	Full text read; purified-enzyme kinetics on adenosine. The adenosine activity is secondary, but it does support the generic node term, which suits an amidohydrolase clade deaminating different adenine compounds.	Xcc2270 Q8P8H1, MJ1541 Q58936, Tm0936 Q9X034, Bc1793 Q81F14	Yes