Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Combined Automated Annotation using Multiple IEA Methods
The novel fission yeast (1,3)beta-D-glucan synthase catalytic subunit Bgs4p is essential during both cytokinesis and polarized growth.
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bgs4+, cwg1+ and orb11+ are the same gene; Bgs4 is the only Bgs subunit shown to be part of the GS enzyme and is essential for integrity during cytokinesis and polarized growth.
"Bgs4p is the only subunit found to be a part of the GS enzyme and essential for maintaining cell integrity during cytokinesis and polarized growth"
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Bgs4 arrives at the septum after Bgs1 and Calcofluor-stained septum material, consistent with a late role in secondary septum synthesis; it needs the medial ring but not the SIN.
"suggesting that Bgs4p is involved in a late process of secondary or general septum synthesis"
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Bgs4 localizes to growing ends, medial ring and septum and to all wall-synthesis sites in sexual differentiation.
"Bgs4p localizes to the growing ends, the medial ring and septum, and at each stage of wall synthesis or remodeling that occurs during sexual differentiation"
ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
Reorganization of the growth pattern of Schizosaccharomyces pombe in invasive filament formation.
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Bgs4 concentrates at the single growing tip of invasive filament cells.
"comprising Spo20, Bgs4, activated Cdc42, and Crn1, are all concentrated at the growing tip"
Extracellular cell wall β(1,3)glucan is required to couple septation to actomyosin ring contraction.
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Bgs4 makes the main cell wall branched beta(1,3)glucan and carries the major GS activity.
"Bgs4 is responsible for the cell wall B-BG synthesis and the major β(1,3)GS activity."
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Without Bgs4 there is no secondary septum and the primary septum edge is defective.
"Bgs4 absence causes the total absence of SS."
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Bgs4-derived glucan is required to keep the actomyosin ring positioned and to couple septum synthesis to ring contraction.
"We show that Bgs4-derived β(1,3)glucan is required for correct and stable actomyosin ring positioning in the cell middle"
A formin-nucleated actin aster concentrates cell wall hydrolases for cell fusion in fission yeast.
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Bgs4 decorates the whole shmoo tip rather than the fusion focus.
"the cell wall synthases Bgs1 and Bgs4 do not concentrate at the fusion focus but rather decorate the entire shmoo tip throughout the fusion process"
A role for F-BAR protein Rga7p during cytokinesis in S. pombe.
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The F-BAR RhoGAP Rga7 is needed for timely transport of Bgs4 from late Golgi to the plasma membrane next to the contractile ring; delayed Bgs4 delivery causes septal defects and lysis.
"does participate in the transport of a β-glucan synthetase (Bgs4p) from the late Golgi compartments to plasma membrane that is adjacent to the contractile ring"
Oligomerization but Not Membrane Bending Underlies the Function of Certain F-BAR Proteins in Cell Motility and Cytokinesis.
Traffic Through the Trans-Golgi Network and the Endosomal System Requires Collaboration Between Exomer and Clathrin Adaptors in Fission Yeast.
Local and global Cdc42 guanine nucleotide exchange factors for fission yeast cell polarity are coordinated by microtubules and the Tea1-Tea4-Pom1 axis.
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mCherry/GFP-Bgs4 marks the plasma membrane of growing tips and becomes mobile patches when polarity is lost.
"Interestingly, during PORTLI growth, Bgs4 appears as transient and mobile patches on the plasma membrane instead of being distributed homogeneously."
Echinocandin Drugs Induce Differential Effects in Cytokinesis Progression and Cell Integrity.
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Bgs4 synthesizes the branched beta(1,3)-D-glucan and carries most GS activity.
"It synthesizes the branched β(1,3)-D-glucan and is responsible for most GS activity"
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Micafungin selectively inhibits Bgs4; bgs4 point mutants (pbr1) are resistant.
"These results show that micafungin selectively inhibits the function of Bgs4, whereas Bgs1 and Bgs3 are not affected by this antifungal."
Papulacandin B resistance in budding and fission yeasts: isolation and characterization of a gene involved in (1,3)beta-D-glucan synthesis in Saccharomyces cerevisiae.
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pbr1 papulacandin resistance resides in the particulate (catalytic) fraction of the S. pombe glucan synthase.
"the resistance character of pbr1 mutants resides in the particulate fraction of the enzyme"
A bipartite glucan synthase-remodeler module organizes branched glucan assembly in the fungal cell wall.
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Ghs2 does not co-immunoprecipitate with Bgs4, and Ghs2 localization is unaffected in the cwg1-1 bgs4 mutant; Bgs4 localization is unaffected in ghs2-2.
"In contrast, GFP-Ghs2 localization was unaffected in bgs4 (cwg1-1) and bgs1 (cps1-191) mutants"
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Bgs4 contributes to beta-1,3-glucan synthesis in septum and lateral wall and associates with the intracellular protein Smi1.
"Bgs4 contributes to β-1,3-glucan synthesis in both the septum and lateral cell wall"
The Fission Yeast Cell Integrity Pathway: A Functional Hub for Cell Survival upon Stress and Beyond.
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Rho1 is the regulatory subunit that activates the Bgs catalytic subunits.
"the essential GTPase Rho1 is the β-GS regulatory subunit responsible for the activation of GS catalytic subunits Bgs1 to Bgs4"
The fission yeast cell wall stress sensor-like proteins Mtl2 and Wsc1 act by turning on the GTPase Rho1p but act independently of the cell wall integrity pathway.