Arginine degradation in Pseudomonas aeruginosa mutants blocked in two arginine catabolic pathways.
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aguA and aguB encode agmatine deiminase and N-carbamoylputrescine amidohydrolase and were mapped in agmatine-utilization mutants.
"The genes encoding agmatine deiminase (aguA) and N-carbamoylputrescine amidinohydrolase (aguB) were 98% cotransducible"
Identification of the putrescine biosynthetic genes in Pseudomonas aeruginosa and characterization of agmatine deiminase and N-carbamoylputrescine amidohydrolase of the arginine decarboxylase pathway.
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Purified AguB is specific for N-carbamoylputrescine.
"AguB was specific to N-carbamoylputrescine and the K(m) and K(cat) values of the enzyme for the substrate were 0.5 mM and 3.3 s(-1), respectively."
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AguB is a nitrilase-family enzyme using thiol-assisted catalysis.
"AguB is a protein of the nitrilase family that performs thiol-assisted catalysis."
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AguA and AguB act in putrescine biosynthesis; an aguAB speC mutant is a putrescine auxotroph.
"a double mutant of aguAB and speC was a putrescine auxotroph."
Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Electronic Gene Ontology annotations created by transferring manual GO annotations between related proteins based on shared sequence features
Electronic Gene Ontology annotations created by ARBA machine learning models