Gene Ontology annotation through association of InterPro records with GO terms
Electronic Gene Ontology annotations created by ARBA machine learning models
Widespread macromolecular interaction perturbations in human genetic disorders.
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High-throughput Y2H screen examining disease mutation effects on PPIs
"Here we functionally profile several thousand missense mutations across a spectrum of Mendelian disorders using various interaction assays."
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KCTD4 detected in interaction assays but not a focus of study
"Approximately 60% of disease-associated missense mutations perturb PPIs"
An inter-species protein-protein interaction network across vast evolutionary distance.
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Yeast-human inter-interactome mapping study
"We systematically probed the yeast and human proteomes for interactions between proteins from these two species"
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KCTD4 detected in screens but not specifically characterized
"we identified 1,583 inter-species interactions between 566 yeast and 471 human proteins"
A reference map of the human binary protein interactome.
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HuRI project providing systematic binary PPI reference map
"Here we present a human 'all-by-all' reference interactome map of human binary protein interactions, or 'HuRI'."
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KCTD4 detected in systematic Y2H screens
"With approximately 53,000 protein-protein interactions, HuRI has approximately four times as many such interactions as there are high-quality curated interactions from small-scale studies."
Deep research review of KCTD4 function
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KCTD4 predicted to form pentameric assembly
"Structural clustering (AlphaFold/analysis) groups KCTD4 in a pentameric cluster (Cluster 4)."
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KCTD4 unlikely to form stable Cul3 complex
"KCTD4 predicted NOT to form a stable Cul3 complex (AlphaFold models gave a 'meaningless complex' / poor PAE)."
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BTB/T1 domain mediates homo-oligomerization
"KCTDs are intracellular proteins whose N-terminal BTB/T1 domain is structurally related to BTB/POZ folds and adapted to drive homo-oligomerization"
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KCTD4 can hetero-oligomerize with KCTD5
"Experimental cell-based interaction assays (co-IP, BRET) demonstrate KCTD5 hetero-oligomerization across family members, including KCTD4 as a detected partner."
Cyberian deep research on KCTD4 function