Annotation inferences using phylogenetic trees

Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt

GO_REF:0000120

Combined Automated Annotation using Multiple IEA Methods

PMID:21478858

TFG-1 function in protein secretion and oncogenesis.

ZK512.5 encodes the C. elegans Sec16 homolog, named SEC-16.

"we identified a Sec16 homolog in C. elegans encoded by ZK512.5, which we named sec-16"
SEC-16 binds TFG-1 directly.

"These data confirm a direct biochemical association between SEC-16 and TFG-1."
SEC-16 localizes to ER exit sites with TFG-1 and COPII.

"We conclude that TFG-1 localizes to ER exit sites with both SEC-16 and COPII machinery."
SEC-16 is required for TFG-1 and SEC-13 puncta at ERES.

"Depletion of SEC-16 led to nearly complete loss of puncta labeled by TFG-1, and the few structures that were observed appeared abnormally large and did not co-stain for the COPII component SEC-13"
SEC-16 is required for embryo production, like COPII subunits.

"7 out of 19 gene products were necessary for embryo production"

PMID:23840591

The ArfGEF GBF-1 Is Required for ER Structure, Secretion and Endocytic Transport in C. elegans.

SEC-16 marks ERES juxtaposed to GBF-1 and the cis-Golgi; its localization is unaffected by gbf-1 RNAi.

"GBF-1 was localized juxtaposed to the ERES marker SEC-16."

file:worm/sec-16A.1/sec-16A.1-uniprot.txt

UniProt entry P34643 (SC161_CAEEL)

file:worm/sec-16A.1/sec-16A.1-deep-research-manual.md

Manual literature review for sec-16A.1

file:worm/sec-16A.1/sec-16A.1-deep-research-wormbase.md

WormBase gene description for sec-16A.1 (worm)