Gene Ontology annotation through association of InterPro records with GO terms
Electronic Gene Ontology annotations created by transferring manual GO annotations between related proteins based on shared sequence features
Combined Automated Annotation using Multiple IEA Methods
UniProtKB entry for pqqE (PqqA peptide cyclase)
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"Catalyzes the cross-linking of a glutamate residue and a"
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"tyrosine residue in the PqqA protein as part of the biosynthesis of"
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"Interacts with PqqD. The interaction is necessary for activity"
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"Belongs to the radical SAM superfamily. PqqE family."
QuickGO GOA annotations for pqqE
Demonstration That the Radical S-Adenosylmethionine (SAM) Enzyme PqqE Catalyzes de Novo Carbon-Carbon Cross-linking within a Peptide Substrate PqqA in the Presence of the Peptide Chaperone PqqD.
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"PqqE, in conjunction with PqqD, carries out the first step in PQQ biosynthesis"
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"radical-mediated formation of a new carbon-carbon bond between two amino acid side chains on PqqA"
Falcon deep research report for pqqE (Q88QV8), Pseudomonas putida KT2440
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"PqqE is the initiating enzyme"
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"catalyzing the first committed chemical transformation"
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"formation of a new intramolecular carbon–carbon bond within PqqA between a conserved glutamate and tyrosine"
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"it uses a **[4Fe–4S] cluster** to reductively cleave **S-adenosyl-L-methionine (SAM)** to generate a **5′-deoxyadenosyl radical"
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"One-electron reduction of the RS [4Fe–4S] cluster enables SAM cleavage to methionine + 5′-dAdo•"
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"PqqE belongs to the **RS-SPASM subfamily**, characterized by additional Fe–S clusters in a C-terminal SPASM domain"
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"AuxII:** a **[4Fe–4S]** cluster ligated by **3 Cys + 1 Asp"
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"PqqD is required** for productive catalysis"
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"The ternary PqqA–PqqD–PqqE complex forms"
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"the most parsimonious localization for catalytic action is **cytosolic**"
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"The direct substrate is the **PqqA precursor peptide** (as a PqqD-bound complex), and the chemical transformation couples **glutamate and tyrosine side chains**"
OpenScientist gene research for pqqE (Q88QV8)