file:PSEPK/grpE/grpE-deep-research-falcon.md

Deep research report for grpE (Q88DU1 / PP_4728) in Pseudomonas putida KT2440

Synthesis of GrpE structure/function (dimeric NEF for DnaK), and KT2440-specific evidence that GrpE is upregulated among general-stress proteins under phenol stress; Pseudomonas grpE-dnaK-dnaJ chaperone gene cluster context.

GO_REF:0000002

Gene Ontology annotation through association of InterPro records with GO terms

GO_REF:0000118

TreeGrafter-generated GO annotations

GO_REF:0000120

Combined Automated Annotation using Multiple IEA Methods

PMID:38110031

New insights into the structure and function of the complex between the Escherichia coli Hsp70, DnaK, and its nucleotide-exchange factor, GrpE

GrpE is the dimeric nucleotide exchange factor for DnaK; binding to the DnaK nucleotide-binding domain opens the nucleotide-binding cleft (movement of subdomain IIB), accelerating ADP release. Its coiled-coil is thermolabile.

PMID:38253530

Structure of the M. tuberculosis DnaK-GrpE complex reveals how key DnaK roles are controlled

Cryo-EM reveals an asymmetric 1:2 DnaK:GrpE complex; the GrpE dimer "ratchets" to remodel both the nucleotide-binding and substrate-binding domains of DnaK, coupling ADP release to substrate release.

PMID:16109938

The heat shock genes dnaK, dnaJ, and grpE are involved in regulation of putisolvin biosynthesis in Pseudomonas putida PCL1445

In P. putida PCL1445, grpE, dnaK, and dnaJ are genomically linked (dnaK downstream of grpE, upstream of dnaJ), supporting a conserved Pseudomonas KJE chaperone gene cluster.