Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on curation of immunofluorescence data
Gene Ontology annotation of human sequence-specific DNA binding transcription factors (DbTFs) based on the TFClass database
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
Mesp1 acts as a master regulator of multipotent cardiovascular progenitor specification.
Induction of MesP1 by Brachyury(T) generates the common multipotent cardiovascular stem cell.
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Brachyury directly binds and activates the MesP1 promoter via a T element; knockdown reduces MesP1 and cardiac markers.
"We finally defined a 3.4 kb proximal MesP1-promoter fragment which is directly bound and activated by Brachyury(T) via a T responsive element as shown via bandshift, chromatin immuneprecipitation, and reporter assays."
Brachyury and related Tbx proteins interact with the Mixl1 homeodomain protein and negatively regulate Mixl1 transcriptional activity.
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Brachyury binds the Mixl1 homeodomain and represses Mixl1 transactivation.
"luciferase reporter experiments indicated that association of Mixl1 with Brachyury and related T-box factors inhibited the transactivating potential of Mixl1 on the Gsc and Pdgfrα promoters."
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Brachyury is a transcriptional activator expressed in nascent mesoderm, tailbud and notochord.
"Brachyury (T), the founding member of the T-box (Tbx) family, is a transcriptional activator and is expressed throughout the nascent mesoderm, tailbud and notochord"
Brachyury, a driver of the epithelial-mesenchymal transition, is overexpressed in human lung tumors: an opportunity for novel interventions against lung cancer.
Mutations in the T (brachyury) gene cause a novel syndrome consisting of sacral agenesis, abnormal ossification of the vertebral bodies and a persistent notochordal canal.
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Homozygous T-box H171R reduces DNA binding and causes sacral agenesis with persistent notochordal canal.
"The homozygous mutation results in diminished DNA binding, increased cell growth, and interferes with the normal expression of genes involved in ossification, notochord maintenance and axial mesoderm development."
Impact of cytosine methylation on DNA binding specificities of human transcription factors.
The human homolog T of the mouse T(Brachyury) gene; gene structure, cDNA sequence, and assignment to chromosome 6q27.
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T is required for posterior mesoderm and axial development; mouse mutants have abnormal notochord and posterior somites.
"Brachyury mutant mice, which lack T protein, die in utero with abnormal notochord, posterior somites, and allantois."
Early evolution of the T-box transcription factor family.
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Brachyury is the most ancient T-box class; the family diversified at the origin of Metazoa.
"Our data confirm that Brachyury is the most ancient member of the T-box family and establish that the T-box family diversified at the onset of Metazoa."
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No T-box genes in sequenced choanoflagellates; interpreted as secondary loss.
"We did not identify T-box genes in either of the two sequenced choanoflagellates (the colonial Salpingoeca rosetta and the unicellular Monosiga brevicollis ), confirming that T-box genes were also lost in this group ( 16 )."
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Capsaspora Brachyury partially rescues dominant-negative XBra in Xenopus.
"Our data show that C. owczarzaki Brachyury ( CoBra ) can partially rescue Xenopus laevis embryos injected with a dominant negative XBra construct."
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Capsaspora Brachyury lacks the target specificity of metazoan Brachyury.
"Through experiments in Xenopus , we demonstrate that C. owczarzaki Brachyury lacks, however, the target specificity displayed by metazoan Brachyury homologs, including sponges."
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Capsaspora Brachyury DNA-binding motif resembles mouse T.
"Our results indicate that CoBra has a highly similar motif to that determined in the mouse Bra-homolog, called T ( Fig. 4 ) ( 28 , 34 – 36 )."