Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Automatic assignment of GO terms using logical inference, based on on inter-ontology links
Gene Ontology annotation of human sequence-specific DNA binding transcription factors (DbTFs) based on the TFClass database
A role for Gbx2 in repression of Otx2 and positioning the mid/hindbrain organizer.
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Mouse loss- and gain-of-function experiments establish that Gbx2 positions and sharpens the Otx2 boundary at the midbrain-hindbrain organizer.
"We propose that formation of a normal MHB organizer depends on a sharp Otx2 caudal border and that Gbx2 is required to position and sharpen this border."
Changing requirements for Gbx2 in development of the cerebellum and maintenance of the mid/hindbrain organizer.
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Conditional loss after embryonic day 9 separates an early requirement for cerebellar territory from a continuing requirement for organizer maintenance.
"In contrast to Gbx2 null mutants, mice lacking Gbx2 in rhombomere 1 (r1) after E9 (Gbx2-CKO) are viable and develop a cerebellum."
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Midbrain-hindbrain organizer gene expression remains dependent on Gbx2 after the conditional deletion time point.
"Mid/hindbrain organizer gene expression, however, continues to be dependent on Gbx2."
Gbx2 and Fgf8 are sequentially required for formation of the midbrain-hindbrain compartment boundary.
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Fate mapping and genetic loss show that Gbx2 specifies hindbrain identity and establishes lineage separation at the midbrain-hindbrain boundary.
"Without Gbx2, hindbrain-born cells abnormally populate the entire midbrain, demonstrating that Gbx2 is essential for specifying hindbrain fate."
Elongation factor 1 alpha1 and genes associated with Usher syndromes are downstream targets of GBX2.
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GBX2 directly binds cis-regulatory sequences at multiple target genes.
"We show through gel shift analyses that sequences within the promoter or introns of EEF1A1, ROBO1, PCDH15, USH2A and NOTCH2, are directly bound by GBX2."
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GBX2 can activate transcription through the EEF1A1 promoter.
"Furthermore, we show that GBX2 activates transcriptional activity through the promoter of EEF1A1"
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Expressed GBX2 fusion protein localizes to nuclei in human cells.
"Merge displays nuclear localization of GFP-GBX2 fusion proteins."
Impact of cytosine methylation on DNA binding specificities of human transcription factors.
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The study systematically measured DNA-binding specificity for hundreds of human transcription factors, including the GBX2 GOA IDA record.
"By analysis of 542 human TFs with methylation-sensitive SELEX (systematic evolution of ligands by exponential enrichment)"
The Temporal Contribution of the Gbx2 Lineage to Cerebellar Neurons.
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Temporally marked Gbx2-expressing precursors contribute to several major cerebellar neuronal classes.
"The Gbx2 lineage gives rise to Purkinje cells, granule neurons, and deep cerebellar neurons across these marking stages."
Characterization and sequence analysis of the human homeobox-containing gene GBX2.
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The human GBX2 homeodomain is identical to mouse Gbx2 and GBX2 transcript is detected in the developing human CNS.
"A single 2.2-kb transcript was detected by Northern analysis in the developing human CNS as well as in other tissues."
The homeobox gene GBX2, a target of the myb oncogene, mediates autocrine growth and monocyte differentiation.
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Ectopic GBX2 expression changes differentiation and growth-factor dependence in myeloblasts.
"Ectopic expression of GBX2 in growth factor-dependent myeloblasts induces monocytic features and independence from exogenous cytokines"