Gene Ontology annotation through association of InterPro records with GO terms

Use of the ND evidence code for Gene Ontology (GO) terms

Annotation inferences using phylogenetic trees

Combined Automated Annotation using Multiple IEA Methods

Sequence analysis of the right end of chromosome XV in Saccharomyces cerevisiae: an insight into the structural and functional significance of sub-telomeric repeat sequences.

The enolase-related sequence shows 61% and 60% DNA sequence identity to Enolases 1 and 2 respectively; it appears species-specific with three copies found in all S. cerevisiae strains studied, at conserved genomic locations.

"The first of these shows 61% and 60% DNA sequence identity to Enolases 1 and 2 respectively. The Enolase-like sequence appears to be species specific, with three copies being found in all strains of S. cerevisiae studied. The location of the three copies is the same for all strains."

file:yeast/ERR1/ERR1-uniprot.txt

UniProt entry P0CX10 (ERR1_YEAST) with inline sequence/domain analysis

UniProt annotates a complete enolase two-domain architecture (Enolase_N PF03952, Enolase_C PF00113; PANTHER PTHR11902) with EC 4.2.1.11, Mg2+ cofactor, and by-similarity catalytic residues ACT_SITE 212 (proton donor) and 346 (proton acceptor); protein existence level is PE 3 (inferred from homology). The record maps both YOR393W and YPL281C mRNAs to this accession, i.e. ERR1 and ERR2 share an identical protein sequence.

"SIMILARITY: Belongs to the enolase family."
Inline sequence comparison (this session): ERR1 is 437 aa with no indels relative to yeast enolases; identity is 100% to ERR2 (P0CX11), ~99.5% to ERR3 (P42222), 68.0% to ENO1 (P00924) and 67.3% to ENO2 (P00925). All eight annotated catalytic / Mg2+ / substrate-binding residues (E212, K346, H160, E169, D247, E296, D321, K397) are identical between ERR1 and ENO2, so the enolase catalytic machinery is fully intact and ERR1 is not a degenerate pseudoenzyme.

"SIMILARITY: Belongs to the enolase family."