Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
UNC-11, a Caenorhabditis elegans AP180 homologue, regulates the size and protein composition of synaptic vesicles.
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unc-11 encodes the worm AP180 homologue, enriched at presynaptic terminals.
"In neurons, UNC-11 is enriched at presynaptic terminals but is also present in cell bodies."
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UNC-11 is required to localise synaptobrevin but not other synaptic vesicle proteins.
"Furthermore, the UNC-11 protein is required to localize synaptobrevin, but not other synaptic vesicle proteins, to synaptic sites."
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Synaptic membrane is still endocytosed in unc-11 nulls but vesicles are 33% larger.
"In the two null mutants, mean vesicle diameter was 33% larger ( unc-11(e47) 39.1 ± 7.3 nm; unc-11(q358) 40.4 ± 8.0 nm)."
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The UNC-11 C-terminus promotes clathrin assembly in vitro.
"Likewise, the C-terminal region of UNC-11 promotes clathrin assembly in vitro despite minimal sequence similarity with AP180 (Golan, Prasad, Lafer, and Alfonso, unpublished data)."
Ubiquitin and AP180 regulate the abundance of GLR-1 glutamate receptors at postsynaptic elements in C. elegans.
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Ubiquitin-driven loss of GLR-1 from synapses requires unc-11.
"overexpression of ubiquitin decreased the abundance of GLR-1 at synapses and decreased the density of GLR-1-containing synapses, and these effects were prevented by mutations in the unc-11 gene, which encodes a clathrin adaptin protein (AP180)."
A map of the interactome network of the metazoan C. elegans.
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High-throughput Y2H interactome map listing UNC-11 interactions.
"Starting with a subset of metazoan-specific proteins, more than 4000 interactions were identified from high-throughput, yeast two-hybrid (HT=Y2H) screens."
Empirically controlled mapping of the Caenorhabditis elegans protein-protein interactome network.
Shifted Transversal Design smart-pooling for high coverage interactome mapping.
Endocytosis and intracellular trafficking contribute to necrotic neurodegeneration in C. elegans.
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Endocytosis is required for necrotic neurodegeneration; the abstract does not name unc-11.
"Depletion of the key endocytic machinery components dynamin, synaptotagmin and endophilin suppresses necrotic neurodegeneration induced by diverse genetic and environmental insults in C. elegans."
RAB-6.2 and the retromer regulate glutamate receptor recycling through a retrograde pathway.
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GLR-1 endocytosis requires UNC-11 and unc-11 mutations suppress GLR-1 loss in rab-6.2 mutants.
"We analyzed GLR-1::GFP localization in rab-6.2; unc-11 double mutants and found that mutations in unc-11 prevent the decrease in GLR-1::GFP puncta number and fluorescence intensity observed in rab-6.2 mutants (Fig."
SH3 interactome conserves general function over specific form.
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SH3 interactome study listing ITSN-1, SORB-1 and MAGU-1 as UNC-11 binders.
"SH3 interactome conserves general function over specific form."
UniProt entry Q9XZI6 (unc-11) subcellular location and domain annotation
Deep research report (falcon/Edison) on C. elegans unc-11
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Deep research adds UNC-11 PI(4,5)P2-liposome binding, actin-coupling condensates of the assembly domain, quantitative electrophysiology and ATG-9 sorting (2023-2026 studies not in GOA).
"Full-length protein associated with 50-nm liposomes containing 2% PIP₂ and bridged them to F-actin, whereas phosphatidylcholine-only liposomes showed minimal association."