tpbA (PA3885) review notes

Literature search (2026-11-05)

No deep-research provider was available. PubMed MCP and Europe PMC search
endpoints failed during this session. Sources read: PMID:19543378 (full text,
Ueda and Wood 2009), PMID:25909591 (full text, Xu et al. 2015), and
PMID:21045298 (crystallisation note, abstract only, not used for claims).

Findings

Purified TpbA-cHis hydrolyses pNPP and two phosphotyrosine peptides; activity is
inhibited by orthovanadate PMID:19543378.
Periplasmic by fractionation PMID:19543378.
Model: TpbA dephosphorylates TpbB (YfiN) and lowers c-di-GMP PMID:19543378.
PAO1 TpbA structures and preference for TpbB pTyr48 PMID:25909591; dual specificity PMID:25909591.
Strain: PMID:19543378 used PA14 (PA14_13660, UniProt A0A0H2ZFK2). Pairwise
comparison of the UniProt sequences (2026-11-05): both 218 aa, 4 differences
(98.2% identity). PseudoCAP's IDA on the PAO1 gene is a routine strain transfer
and is not filed as a defect.

PseudoCAP IBA-seed assessment

PTN node	node term	n_seeds	leaf IBAs	seed annotation (term, evidence, PMID)	other seeds	IBD stands without this seed?	verdict	rationale
PTN001258896	GO:0016791 phosphatase activity	9	297 (77 taxa)	GO:0004725 protein tyrosine phosphatase activity, IDA, PMID:19543378 (PseudoCAP; also UniProt EXP PMID:25909591)	yeast SIW14 S000005043; Arabidopsis AT1G05000, AT2G32960, AT3G02800, AT5G16480; Q6K461 (rice DSP1); Q4QBX1 (Leishmania); I6WXK4 (M. tuberculosis PtpB)	Yes (eight other seeds, including SIW14 and the plant PFA-DSPs)	SOUND	Purified-enzyme assay with phosphotyrosine peptides, reproduced with PAO1 protein. Seed is more specific than the node term; generic phosphatase activity is the right level for an atypical DUSP clade whose members act on proteins or inositol pyrophosphates.

No GO_CENTRAL defect.