PANTHER family review PTHR45810: IBA propagation assessment for cnp1
Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Manual transfer of GO annotation data to genes by curator judgment of sequence model
Electronic Gene Ontology annotations created by ARBA machine learning models
Requirement of Mis6 centromere connector for localizing a CENP-A-like protein in fission yeast.
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SpCENP-A (Cnp1) is essential for establishing centromere chromatin for equal chromosome segregation and localizes to the nonrepetitive inner centromere in a Mis6-dependent manner.
"the fission yeast homolog SpCENP-A is essential for establishing centromere chromatin associated with equal chromosome segregation. SpCENP-A binding to the nonrepetitious inner centromeres depended on Mis6"
The domain structure of centromeres is conserved from fission yeast to humans.
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Cnp1 associates exclusively with the central core DNA and not the flanking heterochromatic repeats, which are bound by Swi6.
"The centromere-associated proteins, Mis6p and Cnp1p (SpCENP-A), associate exclusively with central core DNA, whereas the Swi6 protein binds the surrounding repeats"
The role of Ppe1/PP6 phosphatase for equal chromosome segregation in fission yeast kinetochore.
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spCENP-A is located to central centromere regions in a Mis6-dependent manner; recorded IntAct interaction (Mis12) underlies the protein binding annotation.
"In fission yeast, spCENP-A is located to central centromere regions in a Mis6-dependent manner"
ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
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Genome-wide YFP localization screen confirming nuclear localization of Cnp1.
"we determined the localization of 4,431 proteins, corresponding to approximately 90% of the fission yeast proteome, by tagging each ORF with the yellow fluorescent protein"
Reconstruction of the kinetochore during meiosis in fission yeast Schizosaccharomyces pombe.
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Cnp1 belongs to the Mis6-like group that remains at the centromere throughout meiosis, forming the structural basis of the centromere.
"Mis6-like group proteins remain at the centromere throughout meiosis"
The kinetochore proteins Pcs1 and Mde4 and heterochromatin are required to prevent merotelic orientation.
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Pcs1 and Mde4 localize to the central core of centromeres where Cnp1 chromatin resides; recorded IntAct interaction (Mis12) underlies the protein binding annotation.
"Both Pcs1 and Mde4 localize to the central core of centromeres."
Plasticity of fission yeast CENP-A chromatin driven by relative levels of histone H3 and H4.
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CENP-A(Cnp1) replaces H3 across the central domain forming the specialized chromatin essential for kinetochore assembly; the H3:H4:CENP-A ratio governs CENP-A deposition and segregation fidelity.
"Within the central domain most histone H3 is replaced by the centromere-specific H3 variant CENP-ACnp1 to form the unusual chromatin that occupies most of the 10–12 kb comprising imr and cnt"
Biphasic incorporation of centromeric histone CENP-A in fission yeast.
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Cnp1/CENP-A is deposited at centromeres in two phases (S and G2), with the S-phase pathway requiring the Ams2 GATA factor.
"the fission yeast Schizosaccharomyces pombe has at least two distinct CENP-A deposition phases across the cell cycle: S and G2"
Fission yeast Scm3: A CENP-A receptor required for integrity of subkinetochore chromatin.
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Scm3 is the HJURP-related CENP-A receptor that physically binds Cnp1 and, with Mis16/Mis18, mediates its assembly into subkinetochore chromatin.
"Scm3(Sp) coaffinity purifies with CENP-A(Cnp1) and associates with CENP-A(Cnp1) in vitro"
Csi1 links centromeres to the nuclear envelope for centromere clustering.
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Cnp1-marked centromeres cluster at the nuclear envelope near the spindle pole body during interphase.
"the centromeres of each chromosome are clustered together and attached to the nuclear envelope near the site of the spindle pole body during interphase"
Myb-domain protein Teb1 controls histone levels and centromere assembly in fission yeast.
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Reducing Teb1 function lowers histone levels and reduces centromeric Cnp1 binding and centromeric silencing.
"we examined localization of the centromeric histone H3 variant Cnp1 and found reduced centromeric binding along with reduced centromeric silencing"
Inner Kinetochore Protein Interactions with Regional Centromeres of Fission Yeast.
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The central domain is occupied by CENP-A nucleosomes (two H4 per particle), with inner-kinetochore proteins enriched throughout; H3 is nearly absent.
"H3 nucleosomes are nearly absent from the central domain, which is occupied by centromere-specific H3 (cenH3 or CENP-A) nucleosomes with two H4s per particle"
Prolyl isomerization of the CENP-A N-terminus regulates centromeric integrity in fission yeast.
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The Cnp1 N-terminal GRANT motif (prolyl isomerization) regulates centromeric targeting; the escort chaperone Sim3 binds the Cnp1 NTD.
"we further identified the SpCENP-A-localizing chaperone Sim3 as a SpCENP-A NTD interacting protein that is dependent on GRANT proline residues"