UniProtKB reviewed entry for CASPL1D1
QuickGO GOA annotations for CASPL1D1
Functional and Evolutionary Analysis of the CASPARIAN STRIP MEMBRANE DOMAIN PROTEIN Family.
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Figure 4A shows ectopically expressed AtCASPL1D1 at the lateral plasma membrane, excluded from the CSD. This distinguishes it from CSD-localizing CASPs.
"AtCASPL1s excluded from the CSD but present at the lateral plasma membrane contain in the corresponding position an Ile (versus AtCASP1 Leu-140; one methyl group missing) and Tyr (versus AtCASP1 Phe-171; one extra hydroxyl group)."
Combined Automated Annotation using Multiple IEA Methods
Use of the ND evidence code for Gene Ontology (GO) terms
Isolation and proteomic characterization of the Arabidopsis Golgi defines functional and novel components involved in plant cell wall biosynthesis.
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Original source of the Golgi HDA annotation; the At4g15610 supplementary assignment remains to be checked.
"A collection of 371 proteins consistently identified in all replicates
has been proposed to represent the Golgi proteome, marking an appreciable
advancement in numbers of Golgi-localized proteins."
Label-free protein quantification for plant Golgi protein localization and abundance.
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Original source of the Golgi HDA annotation; the At4g15610 supplementary assignment remains to be checked.
"In total, 102 Golgi-localized
proteins were quantified."
Putative glycosyltransferases and other plant Golgi apparatus proteins are revealed by LOPIT proteomics.
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Original source of the Golgi/TGN/endosome HDA annotation; the At4g15610 supplementary assignment remains to be checked.
"The application of the classification procedure to the proteins with unknown localization resulted in a list of 197 proteins classified as Golgi/ TGN localized ( Supplemental Table S2 )."
Regulation of a plant aquaporin by a Casparian strip membrane domain protein-like.
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The caspl1d1 caspl1d2 double mutant has a weak, condition-dependent suberization phenotype without a root hydraulic conductivity phenotype; an individual CASPL1D1 mechanism is not established.
"The data suggest a slight negative role for CASPL1D1 and
CASPL1D2 in suberization under control or salt stress conditions, with no major
impact on whole root transport functions."
Directed growth and fusion of membrane-wall microdomains requires CASP-mediated inhibition and displacement of secretory foci.
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Native-promoter fusion analysis detected no endodermal CASPL1D1 expression under the tested conditions; CASPL1D1 was excluded from the subsequent six-CASPL knockout. This is not a CASPL1D1 knockout result.
"However, expression analysis, using fluorescent CASPL fusion proteins in both wild-type and caspQ mutant background revealed either no endodermal expression (1D1) or an onset of expression too late to be responsible for the initiation of the lignin microdomains seen in caspQ mutants (Supplementary Fig. 3)."
Falcon (Edison) deep-research report for CASPL1D1
Lignin-based barrier restricts pathogens to the infection site and confers resistance in plants.
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CASPL1D1 knockdown reduces pathogen-induced lignification; combined CASPL4D1 loss strengthens the defect.
"AvrRpm1 ‐induced lignification was largely reduced in amiCASPL1D1 and caspl4d1 plants but was not affected in casp1‐1 and caspl5b3‐1 plants"
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CASPL1D1 knockdown permits greater growth of avirulent Pseudomonas; Figure 5 also shows less confinement of bacteria and hypersensitive cell death.
"The restricted growth of Pst DC3000 ( AvrRpm1 ) and Pst DC3000 ( AvrRpt2 ) in wild‐type plants was alleviated in amiCASPL1D1 and caspl4d1 plants"
OpenScientist hypothesis adjudication: CASPL1D1 immune-barrier mechanism
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Independent hypothesis analysis supports immune-barrier participation but leaves the specific membrane-organizing molecular activity unresolved. Primary gene-specific knockdown evidence agrees with the retained bacterial-defense process.
"None of these establishes the *direct molecular activity*."
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The study distinguishes CASPL1D1 knockdown from CASPL4D1-specific localization and complementation; a CASPL1D1 null and functional tagged rescue would test the proposed mechanism.
"CASPL1D1 evidence is knockdown-only."