Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Efficient export of the glucose transporter Hxt1p from the endoplasmic reticulum requires Gsf2p
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Gsf2 is an integral ER membrane protein required for ER export of Hxt1 and normal localization of Gal2.
"We show that gsf2 mutants accumulate Hxt1p in the endoplasmic reticulum (ER) and that Gsf2p is a 46-kDa integral membrane protein localized to the ER. gsf2 mutants also display a galactose growth defect and abnormal localization of the galactose transporter Gal2p."
Subcellular localization of the yeast proteome
Specialized membrane-localized chaperones prevent aggregation of polytopic proteins in the ER
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Gsf2 acts with other substrate-specific ER membrane chaperones to prevent aggregation of cognate polytopic membrane protein substrates.
"In cells individually lacking one of these components only their cognate substrates, hexose transporters, phosphate transporters, and chitin synthase-III, respectively, aggregate and consequently fail to exit the ER membrane."
Proteomic analysis of the yeast mitochondrial outer membrane reveals accumulation of a subclass of preproteins
One library to make them all: streamlining the creation of yeast libraries via a SWAp-Tag strategy
Falcon deep research report for GSF2
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Falcon supports GSF2 as an ER-localized, cargo-specific accessory/chaperone factor for selected hexose transporters.
"GSF2 encodes an ER-localized integral membrane accessory/chaperone-like factor that promotes proper folding/assembly and efficient ER exit of a select subset of polytopic hexose transporters, with strongest evidence for Hxt1p and effects on Gal2p."