Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Gene Ontology annotation based on curation of immunofluorescence data
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Combined Automated Annotation using Multiple IEA Methods
Structural analysis of Siah1-Siah-interacting protein interactions and insights into the assembly of an E3 ligase multiprotein complex.
MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis.
A quantitative chaperone interaction network reveals the architecture of cellular protein homeostasis pathways.
High-throughput competitive fluorescence polarization assay reveals functional redundancy in the S100 protein family.
Extensive rewiring of the EGFR network in colorectal cancer cells expressing transforming levels of KRAS(G13D).
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
Quantitative fragmentomics allow affinity mapping of interactomes.
Structure of the S100A6 complex with a fragment from the C-terminal domain of Siah-1 interacting protein: a novel mode for S100 protein target recognition.
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NMR/ITC structure of the Ca2+-loaded S100A6 complex with CACYBP/SIP(189-219) shows S100A6 binds the C-terminal SGS region of SIP in a strictly calcium-dependent manner, with a minimal binding fragment Ser189-Arg219; SIP is described as a scaffold in an SCF-like (SCF-TBL1) E3 ligase that links the Siah-1 module to the Skp1-TBL1 substrate-recruiting module.
S100A6 protein negatively regulates CacyBP/SIP-mediated inhibition of gastric cancer cell proliferation and tumorigenesis.
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Overexpression of CacyBP/SIP inhibits gastric cancer cell proliferation and tumorigenesis, an effect strengthened by deleting the S100-binding domain; S100 binding negatively regulates this anti-proliferative activity through reduction of beta-catenin protein and Tcf/LEF transcriptional activity, and the beta-catenin reduction is proteasome-dependent (reversed by MG132).
The CacyBP/SIP protein is sumoylated in neuroblastoma NB2a cells.
Pan-analysis reveals CACYBP to be a novel prognostic and predictive marker for multiple cancers.
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Integrative pan-cancer analysis reports CACYBP is differentially expressed across many cancer types (upregulated in 14, downregulated in 6), associated with prognosis in 13 cancers and with tumor mutational burden, microsatellite instability, and immune infiltration, with protein-level upregulation validated in paired lung adenocarcinoma specimens.