The chemoreceptor genes of the waterflea Daphnia pulex - many Grs but no Ors
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D. pulex has 58 gustatory receptors organized into three subfamilies
"These 58 Grs form 3 distinctive subfamilies of 37, 12, and 5 genes, as well as a highly divergent singleton (Gr58)"
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The 2009 phylogenetic/membrane-topology analysis supports a ligand-gated ion-channel hypothesis rather than directly assaying Daphnia GR channel activity.
"They therefore support the hypothesis that these chemoreceptors are not coupled to G-proteins and instead function as ligand-gated ion channels"
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DpuGr29 location
"29scaffold_4:2213168-2214628NCBI_GNO_04003953116213468595th intron is longer"
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Daphnia GRs have conserved 7TM architecture with TYhhhhhQF motif
"These Grs are fairly easily recognized through their somewhat conserved TM7 regions near the C-terminus, which includes a TYhhhhhQF motif in TM7"
Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Deep research review of DpuGr29 gustatory receptor
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Exact target functional data were not located.
"There is no direct gene-level functional characterization (ligand specificity, electrophysiology, or precise cellular expression) published for DpuGr29."
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The provider uses BmGr9 as a mechanistic comparator, with unresolved oligomerization for DpuGr29.
"whether DpuGr29 functions as a homomer or in heteromeric assemblies remains unknown"
Structural basis of ligand specificity and channel activation in an insect gustatory receptor.
OpenScientist focused assessment of DpuGr29 courtship and ligand-gated channel function
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The report recovers the positive courtship provenance node but did not inspect actual PANTHER topology; its absence-based rejection is disputed.
"PTN001080220 placement was confirmed via QuickGO annotation provenance rather than a live PANTHER tree query."
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The report identifies a target family-signature motif, independently reproduced by the local sequence-only check; it does not establish conductance.
"TYLVILYQF"
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The report explicitly limits the channel interpretation to homology and does not identify a target ligand or functional assembly.
"homology-inferred, not demonstrated"
DpuGr29 focused report critical incorporation
DpuGr29 actual PAINT ancestry and donor provenance
DpuGr29 report motif verification
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A reusable scan reproduces TYLVILYQF364-372 in the 379-residue target, with an unrelated-input control. This is not a channel-function experiment.
"one match: TYLVILYQF at residues 364-372 inclusive."
A Drosophila gustatory receptor essential for aversive taste and inhibiting male-to-male courtship.
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Direct Gr33a loss/rescue experiments establish a fly chemosensory contribution to male-male courtship; exact Daphnia circuit details are not established.
"This phenotype was rescued by the Gr33a+ transgene"