eef1db notes (Danio rerio, eukaryotic translation elongation factor 1 delta b; UniProt A0A8M2B7W1)

2026-09-28 — session log (DANRE_DUPLICATION batch 3, random sample)

Deep research: not available for this gene (Edison/Falcon returned 402 Payment Required;
the OpenAI key is invalid). Not attempted, per instructions. No -deep-research-*.md file
exists. Literature searched by hand (see ../eef1da/eef1da-notes.md). No zebrafish-specific
functional paper exists for either paralog.

Accession: A0A8M2B7W1 (TrEMBL, 578 aa, RefSeq models XP_005160975.1/XP_017207982.1,
chromosome 20) holds all 6 GOA rows (IEA/IBA only). Other eef1db entries include short
isoforms Q5SPD1 (274 aa) and Q5SPD0 (298 aa). Paralog eef1da. Human ortholog EEF1D (P29692).
Shared sequence and expression analysis: ../eef1da/eef1da-bioinformatics/RESULTS.md.

Protein

• GEF region 80.7% identical to human (88/109), 89.0% to eef1da; leucine zipper 52.8% to human.
• Long model carries a 281-aa N-terminal segment before the core; 259 positions align to the
  human eEF1BdeltaL extension at 34.7% identity, modestly above a shuffle control (max 31.8%).
  Weak evidence of homology; the model is computational.
• Human background: PMID:36576126
  PMID:8334168
  PMID:42230146

Expression

• Bgee: same 29 entities as eef1da, high scores throughout; slightly higher than eef1da in
  retina, brain, eye, somite and presomitic mesoderm; lower in maternal/early stages.
• ZFIN: whole-organism in situ, Thisse high-throughput (ZDB-PUB-040907-1).

Annotation decisions

• All 6 rows ACCEPT, identical to eef1da (same PAINT node PTN000174394, same InterPro mappings,
  conserved catalytic region).