NDE1 (YMR145C, UniProt P40215) notes

Module: oxphos (external NDH-2, ndh2_external_activity). Absent from YeastCyc PWY3O-188 gene list.

Evidence journal

• Major external NADH dehydrogenase: nde1 3-fold lower mitochondrial NADH oxidation; nde1 nde2 abolishes it PMID:9733747.
• Same conclusion with gene named NDH1 PMID:9696750; nde1 slows growth on non-fermentable carbon incl. ethanol.
• Location: IMS [UniProt:P40215]; part of a supercomplex of IMS-facing dehydrogenases PMID:11502169.
• Second topomer spans outer membrane, cytosol-exposed, triggers death PMID:31668496.
• nde1 nde2 chemostat glucose metabolism remains respiratory PMID:9733747 -> "pyruvate fermentation to ethanol" IMP rows are indirect; MARK_AS_OVER_ANNOTATED.

Curation decisions

• Core MF GO:0120555, BP GO:0006120 (added as NEW, IMP PMID:9733747; GOA has it for NDI1 only), CC inner membrane (IMS face).
• Apoptosis regulation kept as non-core (topomer-specific moonlighting).