Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Functional genomics of the cilium, a sensory organelle.
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dyf-13 (C27H5.7a) encodes a ciliary protein that undergoes intraflagellar transport; its ciliary localization and transport depend on other IFT and BBS genes, and it is a novel core IFT component required for cilia function.
"One of these, C27H5.7a, encodes a ciliary protein that undergoes IFT."
Dynein-Driven Retrograde Intraflagellar Transport Is Triphasic in C. elegans Sensory Cilia.
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dyf-13 is a component of IFT complex B (identified by affinity purification and mass spectrometry); disruption of the IFT-B complex abolishes ciliary localization of the dynein-2 heavy chain.
"Disruption of the dynein-2 tail domain, light intermediate chain, or intraflagellar transport (IFT)-B complex abolishes dynein-2's ciliary localization, revealing their important roles in ciliary entry of dynein-2."
The Caenorhabditis elegans nephrocystins act as global modifiers of cilium structure.
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DYF-13::GFP is used as an intraflagellar-transport reporter and localizes along amphid channel cilia; the dyf-13 mutant occasionally lacks the IFT-B cargo OSM-6 in amphid distal segments, consistent with an anterograde/IFT-B role.
"The dyf-13 mutant, similar to nphp-4 animals, occasionally lacks OSM-6"
Caenorhabditis elegans DYF-2, an orthologue of human WDR19, is a component of the intraflagellar transport machinery in sensory cilia.
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Establishes the C. elegans dye-filling-defective (dyf) IFT gene class to which dyf-13 belongs; the paralogous dyf-2/WDR19 associates with IFT particle complex B, providing IFT-machinery context for the DYF-13 IFT-B annotations.
"we conclude that DYF-2 can associate with IFT particle complex B"
An essential role for DYF-11/MIP-T3 in assembling functional intraflagellar transport complexes.
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DYF-13 is listed among the conserved C. elegans IFT-associated proteins whose orthologs are enriched in the Chlamydomonas flagellar proteome, supporting its status as a conserved IFT component.
"DYF-3 [34], DYF-13 [35], and IFTA-1 [36]"
Overall Architecture of the Intraflagellar Transport (IFT)-B Complex Containing Cluap1/IFT38 as an Essential Component of the IFT-B Peripheral Subcomplex.
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The mammalian ortholog TTC26/IFT56 was identified as an integral component of the IFT-B core subcomplex, establishing that TTC26/IFT56 (the DYF-13 family) is a genuine IFT-B subunit.
"we identified TTC26/IFT56 and Cluap1/IFT38, neither of which was included with certainty in"