"for the uptake of 2-phenylethanol and for its oxidation to phenylacetic acid via phenylacetaldehyde"

"PqqABCDEF is required for the biosynthesis of pyrroloquinoline quinone (PQQ), a prosthetic group of certain alcohol dehydrogenases that transfers electrons to an independent cytochrome c"

"two quinoprotein alcohol dehydrogenases, a cytochrome c, a periplasmic binding protein, an aldehyde dehydrogenase"

"the two redundant pyrroloquinoline quinone (PQQ)-dependent enzymes PedE and PedH"

"the two redundant pyrroloquinoline quinone (PQQ)-dependent enzymes PedE and PedH"

"We demonstrate that PedH exhibits enzymatic activity only in the presence of lanthanides, including, but not limited to, lanthanum, praseodymium, and cerium"

"this enzyme has a substrate range similar to that of PedE, the recently characterized Ca2+-dependent PQQ-ADH from KT2440"

"The transcription of pedH was found to be strongly influenced by the PedH protein itself, implying a role for PedH as a lanthanide sensory module"

"efficient growth of cells with various naturally occurring alcoholic VOCs relies on the functional production of the lanthanide-dependent ADH PedH"

"a substrate range similar to that of PedE, the recently characterized Ca2+-dependent PQQ-ADH from KT2440"

"the transcription of the genes encoding the Ca2+-utilizing enzyme PedE and the Ln3+-utilizing enzyme PedH are inversely regulated"

"a pedH deletion strain does not grow within 48 h with 2-phenylethanol as the sole carbon source in the presence of a critical concentration of La3+"

"two pyrroloquinoline quinone-dependent ethanol dehydrogenases (PQQ-EDHs) are responsible for the periplasmic oxidation of a broad variety of volatile organic compounds"

"PedH encodes a pyrroloquinoline quinone-dependent alcohol dehydrogenase that is notable for its lanthanide-dependent enzyme activity"

"catalyzes the periplasmic oxidation of a broad range of alcohols – including linear aliphatic alcohols, aromatic alcohols such as 2-phenylethanol, secondary alcohols, and even some aldehydes"

"Its primary structure contains an N-terminal signal peptide followed by a large eight-bladed β-propeller domain that harbors the PQQ prosthetic group"

"PedH belongs to a family of periplasmic **quinoprotein** alcohol dehydrogenases that use the redox cofactor **PQQ** to catalyze oxidation of alcohols (and in some cases aldehydes)."

"In *P. putida* KT2440, PedH and its paralog PedE constitute a **periplasmic oxidation system** important for detoxification and catabolism of volatile alcohols/aldehydes."

"PedH is catalytically active **only in the presence of trivalent lanthanides (Ln3+)** and is inactive with several tested heavy lanthanides/other trivalent metals."

"activity was observed with light-to-mid lanthanides such as **La3+, Ce3+, Pr3+, Nd3+, Sm3+, Gd3+, Tb3+**"

"PedH contains a **conserved active-site Asp** (contrasting with a Ser in PedE) that is associated with **Ln3+ coordination** in lanthanide-dependent quinoprotein dehydrogenases."

"the REE switch is orchestrated by the **PedS2/PedR2** two-component system"

"a **ΔpedH** strain fails to grow on 2-phenylethanol under conditions where the REE switch represses reliance on PedE"

"PedH and PedE were confirmed as the enzymes responsible for dehydrogenation of **(2S,3S)-2,3-butanediol to acetoin**, feeding acetoin into central metabolism via the acetoin dehydrogenase complex."

"Signal peptide present (aa 1-25) for periplasmic export. No transmembrane regions detected in mature protein. Hydrophobic regions at positions 6-27 are part of the signal peptide, not TM helices. All characterized PQQ-ADHs are soluble periplasmic enzymes"

"Comprehensive analysis of PedH function, regulation, and biotechnology applications"

"PedH activity is found with lanthanide concentrations as low as 10 nM and up to 100 uM, with a peak in activity at 1 uM"

"The identity of the electron acceptor/cytochrome partner that re-oxidizes PedH's PQQ in vivo, and the exact routing of electrons into the respiratory chain, were not resolved in the retrieved sources."