Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Mapping organelle proteins and protein complexes in Drosophila melanogaster.
Regulated association of misfolded endoplasmic reticulum lumenal proteins with P58/DNAJc3.
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Mouse P58/DNAJC3 is mostly ER-luminal, binds misfolded proteins through its TPR region, and BiP plus ATP promotes client release via the J-domain HPD histidine H422.
"Most P58 molecules are translocated into the ER lumen, and here we report selective and stable binding to misfolded proteins by P58's TPR-containing N-terminal domain."
Two novel DnaJ chaperone proteins CG5001 and P58IPK regulate the pathogenicity of Huntington's disease related aggregates.
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Overexpressed fly P58IPK co-immunoprecipitated with HTT103Q-GFP in yeast and reduced HTT103Q aggregation in yeast, S2 cells and larval hemocytes.
"HA bands were seen in the input and IP lanes of mutant 103Q with CG5001 and P58IPK whereas the negative controls show no bands in the IP"
DROME/P58IPK/P58IPK-uniprot.txt
P58IPK re-review sequence identity, HPD motif and PANTHER tree placement
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Q9VHA8 is the full-length FlyBase product; fly P58IPK and vertebrate DNAJC3 are orthologs under the Bilateria node despite different PANTHER subfamily labels.
"Q9VHA8 (498 aa) is the FlyBase-linked UniProt entry for FBgn0037718 and is not a fragment or alternative gene model."