Gene Ontology annotation through association of InterPro records with GO terms
Combined Automated Annotation using Multiple IEA Methods
UniProtKB entry Q88KF2 for Pseudomonas putida KT2440 prpD
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UniProt classifies Q88KF2 in the NCBIfam PrpD 2-methylcitrate dehydratase family.
"NCBIfam; TIGR02330; prpD; 1."
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UniProt classifies Q88KF2 in the InterPro PrpD 2-methylcitrate dehydratase family.
"InterPro; IPR012705; 2Me_IsoCit_deHydtase_PrpD."
In vitro conversion of propionate to pyruvate by Salmonella enterica enzymes: 2-methylcitrate dehydratase (PrpD) and aconitase Enzymes catalyze the conversion of 2-methylcitrate to 2-methylisocitrate.
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Purified PrpD has 2-methylcitrate dehydratase activity.
"This work shows that the prpD gene of the prpBCDE operon of this bacterium encodes a protein with 2-methylcitrate dehydratase enzyme activity."
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Purified PrpD lacks an iron-sulfur center and does not require metal or reductant.
"Homogeneous PrpD enzyme did not contain an iron-sulfur center, displayed no requirements for metal cations or reducing agents for activity"
OpenScientist PSEPK 2-methylcitrate-cycle pathway report
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The pathway report assigns the direct dehydration of 2-methylcitrate to 2-methyl-cis-aconitate to PrpD.
"3. Dehydration of 2-methylcitrate → **2-methyl-*cis*-aconitate** — PrpD (direct) **or** AcnD+PrpF (two-component)."
OpenScientist functional-annotation report for PSEPK prpD
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The report identifies the exact target and distinguishes its direct dehydratase reaction from the subsequent aconitase reaction.
"The gene **prpD** (locus tag **PP_2338**; UniProt **Q88KF2**) of *Pseudomonas putida* strain KT2440 encodes **2-methylcitrate dehydratase (EC 4.2.1.79)**"