Gene Ontology annotation through association of InterPro records with GO terms

InterPro2GO maps the GT1 domain to UDP-glycosyltransferase activity; correct but more general than the experimentally supported UDP-glucose donor.

Annotation inferences using phylogenetic trees

PAINT node PTN008730368 propagates only UDP-glucosyltransferase activity; UGT73C5 is a seed of that node.

Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt

Membrane location mapped from an inferred single-pass topology.

GO_REF:0000117

Electronic Gene Ontology annotations created by ARBA machine learning models

ARBA assigns glucosyltransferase activity; correct parent of the supported term.

GO_REF:0000122

AtSubP analysis

Chloroplast prediction without supporting evidence; removed.

PMID:12970342

Detoxification of the Fusarium mycotoxin deoxynivalenol by a UDP-glucosyltransferase from Arabidopsis thaliana.

UGT73C5 (DOGT1) glucosylates deoxynivalenol at C-3 using UDP-glucose, inactivating the toxin; overexpression increases deoxynivalenol tolerance.

"catalyzes the transfer of glucose from UDP-glucose to the hydroxyl group at carbon 3 of deoxynivalenol"

PMID:15342621

N-glucosylation of cytokinins by glycosyltransferases of Arabidopsis thaliana.

In a 105-UGT screen, UGT73C5 formed O-glucosides of trans-zeatin and dihydrozeatin.

"UGT85A1, UGT73C5, and UGT73C1 recognized trans-zeatin and dihydrozeatin"

PMID:15352060

Arabidopsis glycosyltransferases as biocatalysts in fermentation for regioselective synthesis of diverse quercetin glucosides.

Regioselectivity screen of 91 recombinant UGTs on quercetin; UGT73C5 has low 7-O and 4'-O activity (per UniProt).

"discovered 29 that are capable of glucosylating the substrate"

PMID:16214889

The UGT73C5 of Arabidopsis thaliana glucosylates brassinosteroids.

UGT73C5 23-O-glucosylates brassinolide and castasterone; RNAi abolishes BL-23-O-glucoside formation in seedlings; overexpression causes BR deficiency.

"The UGT73C5 of Arabidopsis thaliana catalyses 23-O-glucosylation of the BRs brassinolide (BL) and castasterone"
The authors caution that the inactivation mechanism is not conclusively demonstrated.

"the data do not provide conclusive evidence"
UGT73C5 is one of seven UGT73C genes, six in a tandem array.

"UGT73C5 belongs to a clade of seven genes, six of which are found in a tandem repeat on chromosome 2"

PMID:28887381

Global Analysis of Membrane-associated Protein Oligomerization Using Protein Correlation Profiling.

Proteome-scale microsomal profiling; source of the HDA ER call.

PMID:31519798

Integrative Analysis from the Epigenome to Translatome Uncovers Patterns of Dominant Nuclear Regulation during Transient Stress.

Genome-wide hypoxia/reoxygenation profiling; source of the HEP row.

file:ARATH/UGT73C5/UGT73C5-uniprot.txt

UniProtKB entry Q9ZQ94 (UGT73C5)

Curated function statement summarising brassinosteroid, zeatin, DON and quercetin glucosylation; membrane topology is inferred (ECO:0000305) from a predicted helix.

file:interpro/panther/PTHR48047/PTHR48047-paint.tsv

PAINT annotations for PANTHER family PTHR48047

PTN008730368 carries a single IBD (GO:0035251) seeded by six Arabidopsis UGTs including AT2G36800.