Annotation inferences using phylogenetic trees
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PANTHER phylogenetic propagation transfers the conserved Syo1 symportin/r-protein-carrier functions (protein import into nucleus, ribosomal large subunit biogenesis, unfolded protein binding) across SYO1 orthologues consistent with the experimental yeast data.
Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Global analysis of protein localization in budding yeast.
Rational extension of the ribosome biogenesis pathway using network-guided genetics.
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SYO1 was included among genes whose mutants support roles in ribosome biogenesis.
"confirming involvement of at least 15 new genes"
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The study associated newly confirmed genes with specific ribosomal subunit maturation and trafficking steps.
"new genes with specific aspects of ribosomal subunit maturation, ribosomal particle association, and ribosomal subunit nuclear export"
Synchronizing nuclear import of ribosomal proteins with ribosome assembly.
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Syo1 mediates synchronized nuclear co-import of Rpl5 and Rpl11.
"facilitates synchronized coimport of the two 5S-rRNA binding proteins Rpl5 and Rpl11"
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Syo1 binds Rpl5-Rpl11 and recruits Kap104.
"Syo1 concomitantly binds Rpl5-Rpl11 and furthermore recruits the import receptor Kap104"
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The import complex is released by RanGTP and can be transferred to 5S rRNA.
"The Syo1-Rpl5-Rpl11 import complex is released from Kap104 by RanGTP"
Structure of nascent 5S RNPs at the crossroad between ribosome assembly and MDM2-p53 pathways.
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Structural reconstitution places Syo1 in the conserved hexameric nascent 5S RNP precursor with 5S rRNA, uL18/Rpl5, uL5/Rpl11, Rpf2 and Rrs1.
"This reveals how the nascent 5S rRNA associates with the initial nuclear import complex Syo1-uL18-uL5 and, upon further recruitment of the nucleolar factors Rpf2 and Rrs1, develops into the 5S RNP precursor that can assemble into the pre-ribosome."
Co-translational capturing of nascent ribosomal proteins by their dedicated chaperones.
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Syo1 is one of the dedicated ribosomal-protein chaperones that co-translationally captures specific clients.
"Affinity purification of four chaperones (Rrb1, Syo1, Sqt1 and Yar1) selectively enriched the mRNAs encoding their specific ribosomal protein clients"
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Dedicated chaperones prevent inappropriate interactions and aggregation of ribosomal proteins before assembly.
"Co-translational capturing of nascent ribosomal proteins by dedicated chaperones"
Falcon deep research report on SYO1